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Academic Journal of Medicine & Health Sciences, 2023, 4(8); doi: 10.25236/AJMHS.2023.040805.

Extraction and Analysis of Protein from Unknown Species by SDS-PAGE and Immunobloting

Author(s)

Zeyu Cai

Corresponding Author:
Zeyu Cai
Affiliation(s)

University of Toronto Mississauga, Mississauga, Canada

Abstract

The study of proteins has influenced people’s understanding of the phenomena and activities of life and the nature of life. According to the previous research, examining the proteins composition in the tissue the researchers can identify the unknown species based on its homology to other known proteins and its location within the cell. For this paper, Arctogadus glaciali and Spiniraja whitleyi are used to extract their unknown tissues as samples. Then, SDS-PAGE is used to identify the unknown tissues and the Immunobloting is developed for further detection of individual protein isolates. The result shows that the cod sample from lane 2, 5, 7, and 9 are highly concentrated with myosin light chain proteins, and skates sample from lane 3, 4, 8 and 10 does not contain a significant amount of myosin light chain proteins.

Keywords

SDS-PAGE; Immunobloting; Myosin light chain proteins; Tissue composition

Cite This Paper

Zeyu Cai. Extraction and Analysis of Protein from Unknown Species by SDS-PAGE and Immunobloting. Academic Journal of Medicine & Health Sciences (2023) Vol. 4, Issue 8: 26-30. https://doi.org/10.25236/AJMHS.2023.040805.

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