Screen the therapeutic target of XuanHuang Ointment on acute soft tissue injury by network pharmacology method, and to clarify its mechanism. The main chemical constituents and their targets of XuanHuang Ointment were obtained by using TCMSP database. The disease targets of acute soft tissue injury were searched by GeneCards .A compound-target-disease network was constructed using Cytoscape3.8.2 software. The targets were analyzed by GO analysis and KEGG enrichment analysis based on Metascape database. The core active components of XuanHuang Ointment were Quercetin, beta-sitosterol, Stigmasterol. The core target spots are: NCOA2, PTGS2, PTGS1, PGR and NR3C2 .Pathways in cancer, Lipid and atherosclerosis, AGE-RAGE signaling pathway in diabetic complications may play a potential role in XuanHuang Ointment in the treatment of acute soft tissue injury Signaling pathway. Conclusion XuanHuang Ointment has the characteristics of multiple components, multiple targets and overall regulation in the treatment of acute soft tissue injury.

Rui Hua, Miao Tan, Yan Cheng. Mechanism of Xuanhuang Ointments for the Acute Soft Tissue Injury Based on Network Pharmacology. Frontiers in Medical Science Research (2023) Vol. 5, Issue 10: 137-142. https://doi.org/10.25236/FMSR.2023.051020.

[1] Wang Zhexin. Clinical observation of integrated Chinese and Western medicine [J]. Chinese Chinese Medicine Emergency, 2014, 10:1930-1931. 

[2] Dong F, Xue C, Wang Y, et al. Hydroxysafflor yellow A attenuates the expression of inflammatory cytokines in acute soft tissue injury. Sci Rep. 2017; 7:40584. Published 2017 Jan 11. doi:10. 1038/ srep 40584

[3] Si Yuhao, Ma Yong, Guo Yang, Pan Yalan, Zheng Suyang, Wang Lining, Rui Min. Ma Yong's experience in treating acute tendon injuries by promoting blood circulation and benefiting water method [J]. Chinese Journal of Traditional Chinese Medicine, 2018, 33 (09): 3951-3954. 

[4] Jiang Guopeng, Xie Xingwen, Gu Yubiao, Li Jing, Xu Wei. Progress in TCM treatment of acute soft tissue injury [J]. Chinese Medicine Emergency, 2015, 24 (07): 1209-1211. 

[5] Yuan Daichang, Yuan Ling, Yuan Panpan, Nan Yi. Based on the study of rhubarb taste and efficacy of ancient herbs [J]. Asia-Pacific Traditional Medicine, 2022, 18 (01): 196-202. 

[6] Chen Ruijun, Wang Xueyuan, La Xiaojin, Li Ji'an, Liang Guiding, Cao Huijuan, Wang Yaoyao, Zhang Min. Progress in dandelion medicinal research [J]. Journal of Modern Integrated Traditional Chinese and Western Medicine, 2021, 30 (05): 563-567. 

[7] Yu Wei, Li Miao, Zhang Congwei. Zhang Xichun used frankincense and myrrh experience [J]. The Chinese Journal of Basic Medicine of Traditional Chinese Medicine, 2021, 27(08): 1238-1240. DOI:10. 19945/j. cnki. issn. 1006-3250. 2021. 08. 013. 

[8] Cheng Yan, Zhang Hongwu, Li Yongge, Yan Yong. Xuanhuang ointment treated the swelling after limb fracture [J]. Chinese Journal of TCM orthopedics, 2005 (05): 68-69. 

[9] Yang Zhenxing, Dou Qunli. Clinical study on the treatment of acute soft tissue injury [J]. Asia-Pacific Traditional Medicine, 2017, 13 (02): 144-145. 

[10] Wang Shaoping, Zhao Yimu, Li Panpan, Song Shuyi, Han Fei, Zhang Jiayu, Dai Long. Study on the mechanism of blood breaking and blood stasis based on network pharmacology [J]. Modern Chinese medicine, 2021, 23(03):457-463+469. DOI:10. 13313/j. issn. 1673-4890. 20200219001.

[11] Zhao Guangyao, Zhao Kun, Jiang Wenwen, Wang Daoping, Zhang Ming. Study on the inhibition of α -glucosidase activity based on network pharmacology [J]. Natural Products Research and Development, 2020, 32(03):403-413+440. DOI:10. 16333/j. 1001-6880. 2020. 3. 008.

[12] Zhang Songbao, Kong Lingjie, Gu Wei, Li Chao, Qiu Rongli, Chao Jianguo, Pei Lingfeng, Guo Yanfang. Network pharmacology study of dandelion chemical composition analysis and its anticancer mechanism based on HPLC-Q-TOF-M S/MS technology [J / OL]. Research and development of natural products: 1-16 [2022-03-02]. http://kns. cnki. net/kcms/detail/51. 1335. Q. 20211220. 1407. 002. html. 

[13] Meng Ran, Xue Zhizhong, Lu Xuelin, Wang Xiuping. Progress in the study of the effective components and pharmacological effects of dandelion [J]. Jiangsu Agricultural Science, 2021, 49(09):36-43. DOI:10. 15889/j. issn. 1002-1302. 2021. 09. 006.