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Frontiers in Medical Science Research, 2023, 5(11); doi: 10.25236/FMSR.2023.051101.

Mitofusin 2 protects effect of breviscapine against hepatic ischemia/reperfusion injury: Regulation of endoplasmic reticulum stress and apoptosis


Wei Xu1, Tian Yu2, Yangpeng Ou1, Yao Tian1, Caiyun Chen1, Jin Gong1, Xing Lin2

Corresponding Author:
Xing Lin

1Department of General Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, 510630, China

2Department of General Surgery, Guangzhou Tianhe District People's Hospital, Guangzhou, Guangdong, 510075, China


To investigate the effect of breviscapine against hepatic ischemia/reperfusion (I/R) injury and its underlying mechanisms in rats, 40 Sprague-Dawley male rats were randomly assorted into five groups (n=8 per group) as follows: Sham, I/R1 + normal saline (NS), I/R1 + breviscapine (Bre), I/R2 + NS and I/R2 + Bre, where label 1 and 2 denote ischemia time for 20 and 60 min, respectively. Bre or NS (10 mg/kg) was administered to the tail vein 1 h prior to surgery and immediately postoperatively. Reperfusion for 6 h, the blood and hepatic samples were collected immediately to evaluate hepatic function and histopathological changes, in addition with the expression levels of mitofusin 2 (Mfn2) and endoplasmic reticulum stress (ERS)-associated proteins. The results demonstrated that breviscapine decreased the elevated serum level of ALT and AST and ameliorated histopathological changes compared with the NS groups (P<0.05). The expression of Mfn2 and Glucose-regulated protein 78 (GRP78) and Bcl2 protein were significantly increased in the Bre groups (P<0.05), accompanied by decreasing the expression of C/EBP homologous protein (CHOP) and cleaved caspase-3 (P<0.05) compared with the NS groups. These data revealed that breviscapine could ameliorate the hepatic I/R injury, possibly contribute to the up-regulation of Mfn2 expression to inhibit the ERS pathway, thus reducing the apoptosis of hepatocyte.


Hepatic ischemia/reperfusion; Breviscapine; Mitofusin 2; Endoplasmic reticulum stress; Apoptosis

Cite This Paper

Wei Xu, Tian Yu, Yangpeng Ou, Yao Tian, Caiyun Chen, Jin Gong, Xing Lin. Mitofusin 2 protects effect of breviscapine against hepatic ischemia/reperfusion injury: Regulation of endoplasmic reticulum stress and apoptosis. Frontiers in Medical Science Research (2023) Vol. 5, Issue 11: 1-9. https://doi.org/10.25236/FMSR.2023.051101.


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