Frontiers in Medical Science Research, 2023, 5(12); doi: 10.25236/FMSR.2023.051205.
Hao Zhao1, Xie Li2, Wu Yingying1
1Department of Oncology, The Second Naval Hospital of the Southern Theater Command of PLA, Sanya, China
2Department of Anesthesiology, Qilu Hospital of Shandong University Dezhou Hospital, Dezhou, China
To investigate the effects and mechanisms of SFRP2 on the proliferation and invasion of human gastric cancer cell line BGC823, gastric cancer cell line BGC823 was used, which was resuscitated by liquid nitrogen, added with DMEM medium (containing 10% fetal bovine serum and 1% penicillin-streptomycin), and cultured at 37 ℃ with 5% CO2. When the cell attachment rate exceeded 80%, three passages were performed. The blank control plasmid pc-DNA and SFRP2 overexpression plasmid were transfected with Lipo-fectamine 2000 reagent. BGC823 cells in logarithmic growth phase were divided into control group, blank plasmid group and SFRP2 overexpression group. The cells in each group were cultured for 24 hours, and the proliferation of the cells in each group was detected by MTT assay and clone formation assay. The expression of E-cadherin, β-catenin and SFRP2 was detected by Western blot. The results showed that the proliferative activity of cells in the SFRP2 overexpression group was significantly lower than that of cells in the control and blank plasmid groups (P<0 05); there was no significant difference in the proliferative activity of cells in the control and blank plasmid groups. The numbers of cell clones in the control, blank plasmid and SFRP2 overexpression groups were (255.69 ± 29.71), (243.18 ± 5.82) and (178.28 ± 23.42), respectively; among them, the number of cell clones in the SFRP2 overexpression group was significantly lower than that of the control group and the blank plasmid group (P<0 05).The relative numbers of E-cadherin The relative expression of E-cadherin and SFRP 2 proteins in the SFRP 2 overexpression group was significantly higher than that in the control and blank plasmid groups (P<0.05), and the relative expression of β-catenin protein in the SFRP 2 overexpression group was significantly lower than that in the control and blank plasmid groups (P<0.05). Cells in each experimental group were detected by Transwell invasion assay. The results showed that the number of cells in the SFRP 2 overexpression group was significantly lower than that in the control group and the blank plasmid group (P < 0. 05), and there was no significant difference between the control group and the blank plasmid group.SFRP2 protein can inhibit the WNT pathway and inhibit the occurrence and invasion and metastasis of gastric cancer EMT.
Gastric cancer, SFRP2, E-cadherin, WNT pathway, EMT
Hao Zhao, Xie Li, Wu Yingying. The expression of SFRP2 in gastric cancer and its mechanism of inhibiting gastric cancer invasion and metastasis. Frontiers in Medical Science Research (2023) Vol. 5, Issue 12: 43-48. https://doi.org/10.25236/FMSR.2023.051205.
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