In order to explore the pharmacodynamic material basis and mechanism of Xuanmai Ganjie Decocyion in the treatment of Children’s atopic cough by means of network pharmacology. Through the Herb database, the main chemical components and targets of Xuanmai Ganjie Decoction were searched, supplemented in the combination with the literature. We searched The targets related to AC diseases were obtained through human gene database (Gencards), online mendelian inheritance in man(OMIM). Then we obtained the common targets of active components of drug and disease targets. The protein-protein interaction network of drug and disease common targets was constructed based on String platform, The PPI network was built through Cytoscape 3.7.2, and the cytohubba analyzed the key targets of Xuanmai Ganjie Decoction in the treatment of AC. GO and KEGG enrichment analysis was carried out using the Metascape website, and bubble charts were drawn using the WeScape online website. Resultly, a total of 82 active ingredients and 86 related targets of Xuanmai Ganjie Decoction and 82 related targets of AC were obtained, Cytohubba finally screened out 10 key edges. The enrichment analysis of KEGG pathway mainly involves calcium signaling pathway, chemokine signaling pathway, Th17 cell differentiation and JAK2/STAT3 signaling pathway. The biological process of GO enrichment analysis mainly involves the activation of protein tyrosine kinase. In conclusion, it may act on the chemokine signaling pathway, Th17 cell activation and tyrosine protein kinase activation through targets such as JAK2, STAT3, VEGFA and other targets to play a therapeutic role in AC.

Yile Kou, Hui Ding. Exploration on the Mechanism of Xuanmai Ganjie Decoction in the Treatment of Children’s Atopic Cough Based on Network Pharmacology. Academic Journal of Medicine & Health Sciences (2024), Vol. 5, Issue 1: 51-59. https://doi.org/10.25236/AJMHS.2024.050109.

[1] Chronic Cough Collaborative Group of the Respiratory Group of the Chinese Medical Pediatrics Branch. Editorial Committee of the Chinese Journal of Pediatrics Guidelines for the diagnosis and treatment of chronic cough in children in China (2013 revision)[J]. Chinese Journal of Pediatrics, 2014, 52(3):184-188.

[2] The Asthma Group of the Respiratory Diseases Section of the Medical Association. Guidelines for the diagnosis and treatment of cough (2021) [J]. Chinese Journal of Tuberculosis and Respiratory Diseases, 2022, 45(1):34.

[3] Jones LL, Hashim A, McKeever T, et al. Parental and household smoking and the increased risk of bronchitis, bronchiolitis and other lower respiratory infections in infancy: systematic review and meta-analysis [J]. Respiratory research, 2011, 12(1):5.

[4] Zhang Fan. Literature study of pediatric allergic cough evidence and medication pattern [D]. Hunan University of Traditional Chinese Medicine, 2020. 

[5] The Subspecialty Group of Pharmacology,the Society of Pediatrics,Chinese Medical Association, et al. Clinical practice guidelines for the diagnosis and management of children with cough in China(version 2021) [J]. Chinese Journal of Pediatrics, 2021, 59(09):720-729. 

[6] Lai Kefang, Chen Ruchong, Lin Jiangtao, et al. A Prospective, Multicenter Survey on Causes of Chronic Cough in China[J]. Chest, 2013, 143(3):613-620. 

[7] Julie M. Marchant, Peter A. Newcombe, Elizabeth F. Juniper, et al. What Is the Burden of Chronic Cough for Families? [J]. Chest, 2008, 134(2):303-309.

[8] Su Xuan. Chao Enxiang:Clinical research on the treatment of "wind cough" in traditional Chinese medicine and analysis of related problems [J]. China Medicine and Pharmacy, 2015, 5(21):1-3. 

[9] Zhu Danxi. Danxi Xinfa [M]. Beijing: China Medical Science and Technology Press, 2012. 

[10] Nagata Yuka, Suzuki Ryo, . FcεRI: A Master Regulator of Mast Cell Functions [J]. Cells, 2022, 11(4):622-622. 

[11] Zhu Minghua, Liu Yan, Koonpaew Surapong, et al. Positive and negative regulation of FcepsilonRI-mediated signaling by the adaptor protein LAB/NTAL [J]. The Journal of experimental medicine, 2004, 200(8):991-1000. 

[12] Zhang Zhonglin, Zhong Ling, Zheng Li, et al. Progress in the study of molecular mechanisms of mast cell activation[J]. Medical Journal of the Chinese People's Armed Police Forces, 2014, 25(07):743-746. 

[13] Feng Qing. Effect of hydrogen-saturated sterilized water on Anti-DNP-IgE-induced mast cell activation [D]. Hebei Medical University, 2017. 

[14] Lan Chun, Huang Ting, Wang Mengqing, et al. Treatment of adenoid hypertrophy in children by Xuanmai-Ganjie Tang from the perspective of "prolonged illness injuring yin" [J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy, 2022, 28(03):192-194. 

[15] Choi Jae Ho, Jin Sun Woo, Kim Hyung Gyun, et al. Saponins, especially platyconic acid A, from Platycodon grandiflorum reduce airway inflammation in ovalbumin-induced mice and PMA-exposed A549 cells.[J]. Journal of agricultural and food chemistry, 2015, 63(5):1468-1476.

[16] Zhao Yuanyuan, Zhao Yiying, He Hongjuan, et al. Mechanism of Jiegeng in Improving Asthma:An Exploration Based on Network Pharmacology and Experimental Verification [J]. Pharmacology and Clinics of Chinese Materia Medica, 2021, 37(06):82-89.

[17] Chen Yue. Exploring the effect of Xifeng Dingwang Fang on airway inflammation in asthmatic guinea pigs based on JAK2/STAT3 signaling pathway [D]. Anhui University of Traditional Chinese Medicine, 2021. 

[18] Guan Yan, Xie Qiangmin. Regulation of licorice flavonoids on cytokines mRNA expression and oxidation reaction in mice with lung inflammation [J]. Chinese Traditional and Herbal Drugs, 2009, 40(08):1254-1259. 

[19] Fan Wenjing, Ren Juanning, Zhan Xiujun, et al. Research progress on mechanism of Jiegeng Decoction and its active components in treatment of acute lung injury [J]. Chinese Traditional and Herbal Drugs, 2022, 53(04):1230-1239.

[20] Shan Jinjun, Zou Jiashuang, Xu Jianya, et al. Research Advances of Jiegeng Tang [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(19):304-306. 

[21] Damasceno Luis Eduardo Alves, Prado Douglas Silva, Veras Flavio Protasio, et al. PKM2 promotes Th17 cell differentiation and autoimmune inflammation by fine-tuning STAT3 activation [J]. The Journal of experimental medicine, 2020, 217(10):e20190613. 

[22] Cosmi L, Liotta F, Maggi E, et al. Th17 cells: new players in asthma pathogenesis [J]. Allergy, 2011, 66(8):989-998.

[23] Bai Peng, Han Guizhen, Lu Li. Expressions of CXCR2,CCR1,CCL3 and CCL2 in sputum of children with bronchial asthma and their relationships with pulmonary function and airway inflammation [J]. Hebei Medical Journal, 2019, 41(23):3555-3558. 

[24] Huang Huifen, Fan Liang, Fu Rumei, et al. Efficacy Observation of Addition and Subtraction Therapy of Xuanshen Ganju Tang Combined with Sanniutang to Post-infection Cough with Lung Injury Caused by Dryness and Heat [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019, 25(03):125-130.

[25] Zhu Li, Shen Jingli. The study of relationship between serum chemokine 3,CXC chemokine receptor 4,galactosin-9 levels and pulmonary function and airway inflammation in children with bronchial asthma [J]. Maternal and Child Health Care of China, 2021, 36(17):4067-4070. 

[26] Wang Xinhong, Zhang Chi, Zhou Li, et al. The antitussive and expectorant mechanism of Platycodon total saponins based on metabonomics [J]. Acta Pharmaceutica Sinica, 2022, 57(03):757-765. 

[27] Xu Lingwen, Wang Huabing, Dong Fang, et al. Inhibitory effect of ophiopogonin D pretreatment on LPS-mediated inflammatory and oxidative damage of human pulmonary epithelial Beas-2B cells [J]. Journal of Shenyang Pharmaceutical University, 2021, 38(12):1302-1308.