This study aims to establish an ovarian premature aging rat model induced by cyclophosphamide and explore the effects of CTX on ovarian function and bone microstructure in rats. This study used SD rats as the modeling subjects, divided into a modeling group, a control group, and an aging control group. The ovarian premature aging model was established by intraperitoneal injection of CTX, and vaginal smears were collected for cytological analysis after 14 days. Serum was collected after 14 days for sex hormone determination to verify the success of the model. After successful modeling, femoral head specimens were taken from the rats for histological sectioning, stained with HE, and analyzed for bone microstructure based on the staining results. Mesenchymal stem cells were extracted from the bone marrow of the three groups of rats and labeled with specific markers, then cultured in osteogenic induction medium. Alkaline phosphatase staining was performed after 7 and 21 days to observe the osteogenic differentiation ability of the rats in different groups. Bone microstructure analysis was conducted from the dual perspectives of histological sectioning with HE staining and osteogenic induction differentiation. The results showed that the modeling of ovarian premature aging in rats induced by CTX was successful, with changes in the microstructure of the femoral bone tissue and a decrease in osteogenic differentiation ability.

Dong Jiaqi, Zhang Yanru. Bone microstructure analysis of ovarian premature aging rat model under dual perspectives. Frontiers in Medical Science Research (2024), Vol. 6, Issue 5: 15-19. https://doi.org/10.25236/FMSR.2024.060503.

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