Periodontitis is a chronic inflammatory disease caused by bacterial pathogens, characterized by inflammatory infiltration and progressive alveolar bone loss. This paper provides an overview of the mechanism, methods and treatment of model establishment and provides relevant information for future studies using the ligature-induced periodontitis model in mice.

Meng Li, Jin Zhao. Research progress of ligature-induced periodontitis model in mice. Frontiers in Medical Science Research (2023) Vol. 5, Issue 3: 75-81. https://doi.org/10.25236/FMSR.2023.050313.

[1] Bai L, Chen B Y, Liu Y, et al. A Mouse Periodontitis Model with Humanized Oral Bacterial Community. Frontiers in Cellular and Infection Microbiology, (2022), 12:842845.  

[2] Liu Q, Guo S, Huang Y, et al. Inhibition of TRPA1 Ameliorates Periodontitis by Reducing Periodontal Ligament Cell Oxidative Stress and Apoptosis via PERK/eIF2α/ATF-4/CHOP Signal Pathway. Oxid Med Cell Longev,(2022),2022:4107915.

[3] Nakajima M ,Tanner E , Nakajima N , et al. Topical treatment of periodontitis using an iongel. Biomaterials, (2021), 276:121069.

[4] Lin M K, Li L L, Yan F H. Research progress on the relationship between prediabetes and periodontitis. Chinese Journal of Stomatology, (2022), 57(6):6.

[5] Wang Xing. Report of the fourth national oral health epidemiological survey [M]. Beijing:People's Health Publishing House, 2018: 40.

[6] Sanz M, Marco Del Castillo A, Jepsen S,et al. Periodontitis and cardiovascular diseases: Consensus report. J Clin Periodontol, (2020) ,47(3):268-288. 

[7] Baeza M, Morales A, Cisterna C,et al. Effect of periodontal treatment in patients with periodontitis and diabetes: systematic review and meta-analysis. Journal of Applied Oral Science, (2020), 28:e20190248. 

[8] Bui FQ, Almeida-da-Silva CLC, Huynh B, et al. Association between periodontal pathogens and systemic disease. Biomed J, (2019) ,42(1):27-35. 

[9] Wu Y H, Taya Y,Kuraji R, et al. Dynamic microstructural changes in alveolar bone in ligature‐induced experimental periodontitis. Odontology, (2020), 108(3):339-349.

[10] Marchesan J, Girnary MS, Jing L,et al.An experimental murine model to study periodontitis.. Nature protocols, (2018), 13(10):2247-2267.

[11] Yoon H, Jung B H, Yoo K Y, et al. Temporal changes of periodontal tissue pathology in a periodontitis animal model. Journal of Periodontal & Implant Science, (2022), 52. 

[12] Ai R, Li D, Shi L, et al. Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model. Frontiers in Microbiology, (2022), 13:875091. 

[13] Chipashvili O, Bor B. Ligature-induced periodontitis mouse model protocol for studying Saccharibacteria. STAR protocols, ( 2022), 3(1): 101167.

[14] Xiao E, Mattos M, Vieira GHA,et al. Diabetes Enhances IL-17 Expression and Alters the Oral Microbiome to Increase Its Pathogenicity. Cell Host Microbe. (2017), 22(1):120-128.

[15] Abe T, Hajishengallis G. Optimization of the ligature-induced periodontitis model in mice. Journal of Immunological Methods, (2013), 394(1-2):49-54.

[16] Li H,Zhong X,Li W,et al.Effects of 1,25-dihydroxyvitamin D3 on experimental periodontitis and AhR/NF-κB/NLRP3 inflammasome pathway in a mouse model.Journal of applied oral science, (2019), 27(8):8-13.

[17] Lin P, Niimi H, Ohsugi Y, et al. Application of Ligature-Induced Periodontitis in Mice to Explore the Molecular Mechanism of Periodontal Disease. International Journal of Molecular Sciences, (2021), 22(16): 8900.

[18] Li D, Feng Y, Tang H, et al. A simplified and effective method for generation of experimental murine periodontitis model. Frontiers in Bioengineering and Biotechnology, (2020), 8: 444.

[19] Tamura H, Maekawa T, Hiyoshi T, et al.Analysis of Experimental Ligature-Induced Periodontitis Model in Mice. Methods Mol Biol, (2021),2210:237-250.

[20] Fan C, Zhang X, Upton Z. Anti-inflammatory effects of shikonin in human periodontal ligament cells. Pharmaceutical Biology, (2018),56(1):415-421.

[21] Casili G, Ardizzone A, Lanza M, et al. Treatment with Luteolin Improves Lipopolysaccharide-Induced Periodontal Diseases in mice. Biomedicines,(2020),8(10):442-453.

[22] Zhang J, Yu C, Zhang X, et al. Porphyromonas gingivalis lipopolysaccharide induces cognitive dysfunction, mediated by neuronal inflammation via activation of the TLR4 signaling pathway in C57BL/6 mice.Journal of Neuroinflammation,(2018),15(1):37-45.

[23] He W, Xie X, Li C, et al. Adenosine A2A Receptor Antagonist Improves Cognitive Impairment by Inhibiting Neuroinflammation and Excitatory Neurotoxicity in Chronic Periodontitis Mice. Molecules, (2022), 27(19): 6267.

[24] Gustavo M, Francisca C, Pablo I J, et al. Alveolar bone resorption and Th1/Th17-associated immune response triggered during Aggregatibacter actinomycetemcomitans -induced experimental periodontitis are serotype-dependent. Journal of Periodontology, (2018), 89(10):1249–61.

[25] Monasterio G, Budini V, Fernández B, et al. IL-22-Expressing CD4+Ahr+ T Lymphocytes Are Associated With RANKL-Mediated Alveolar Bone Resorption During Experimental Periodontitis. Journal of Periodontal Research,(2019),54(5):513–524. 

[26] de Molon R S, de Avila E D, Boas Nogueira A V, et al. Evaluation of the host response in various models of induced periodontal disease in mice. Journal of periodontology, (2014), 85(3): 465-477.

[27] Tian H, Zhang Z, Wang X, et al. Role of experimental periodontitis in inducing pulmonary inflammation in mice. Oral Diseases, (2022), 28(8): 2294-2303.

[28] Kim S, Bando Y, Chang C, et al. Topical application of Porphyromonas gingivalis into the gingival pocket in mice leads to chronic active infection, periodontitis and systemic inflammation. Int J Mol Med. (2022), 50(2):103. 

[29] Akkaoui J, Yamada C, Duarte C, et al.Contribution of Porphyromonas gingivalis lipopolysaccharide to experimental periodontitis in relation to aging. Geroscience, (2021) ,43(1):367-376. 

[30] Cheat B, Torrens C, Foda A, et al. NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis. Frontiers in immunology, (2022),13: 608.

[31] Nibali L, Gkranias N, Mainas G, et al. Periodontitis and implant complications in diabetes. Periodontology 2000, (2022), 90(1): 88-105.

[32] Chaves I M, Zicker M C, Laranjeira A O, et al. Dysbiotic oral microbiota contributes to alveolar bone loss associated with obesity in mice. Journal of Applied Oral Science, (2022), 30:e20220238.

[33] Chipashvili O, Bor B. Ligature-induced periodontitis mouse model protocol for studying Saccharibacteria. STAR protocols, (2022), 3(1): 101167.

[34] Qi W, Yang X, Ye N, et al.TLR4 gene in the regulation of periodontitis and its molecular mechanism. Experimental and therapeutic medicine, (2019), 18(3):1961-1966. 

[35] Huang H, Pan W, Wang Y, et al. Nanoparticulate cell-free DNA scavenger for treating inflammatory bone loss in periodontitis. Nature Communications, (2022), 13(1): 5925.

[36] Louisya A, Geoffroya V, Halganda B, et al. Interleukin-33 Deficiency Exacerbates Bone Loss Associated with Porphyromonas Gingivalis-Induced Experimental Periodontitis in Female Mice. Cell Physiol Biochem, (2022), 56: 270-281.

[37] Franchin M, Taira T M, da Silva Prado D, et al. PI3Kγ controls IL-17A expression and attenuates alveolar bone loss in an experimental periodontitis model. Inflammation Research,(2022): 1-8.

[38] Zhang J, Lu R, Zou P, et al. Preliminary study on the involvement of platelets in mouse experimental periodontitis. Journal of Dental Sciences, (2022),17(4):1494-1500.

[39] Ishii T, Ruiz-Torruella M, Yamamoto K, et al. Locally Secreted Semaphorin 4D Is Engaged in Both Pathogenic Bone Resorption and Retarded Bone Regeneration in a Ligature-Induced Mouse Model of Periodontitis. International Journal of Molecular Sciences, (2022), 23(10): 5630.

[40] Gong A, Liu Y, Xu F, et al. Role of 1, 25-dihydroxyvitamin D in alleviating alveolar bone loss and gingival inflammation in ligature-induced periodontitis. American Journal of Translational Research, (2022), 14(5): 3079.

[41] Ikeda E, Tanaka D, Glogauer M, et al. Healing effects of monomer and dimer resveratrol in a mouse periodontitis model. BMC Oral Health, (2022), 22(1): 1-6.

[42] Chen Y, Hu Y. Therapeutic potential of PPARα agonist in ligature-induced experimental periodontitis. Journal of Applied Oral Science, (2022), 30:e20210648.

[43] Bae J E, Hwang S M, Aryal Y P, et al. Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model. Frontiers in Physiology, (2022),13: 2117.

[44] Zhou S, Ji Y, Yao H, et al. Application of Ginsenoside Rd in Periodontitis With Inhibitory Effects on Pathogenicity, Inflammation, and Bone Resorption. Frontiers in Cellular and Infection Microbiology, (2022): 387.

[45] Tang G, Ma H, Liu S, et al. Pyrroloquinoline quinone inhibits ligature-induced alveolar bone loss through regulation of redox balance and cell senescence. American Journal of Translational Research, (2022), 14(1): 582.

[46] Barutta F, Bellini S, Durazzo M, et al. Novel Insight into the Mechanisms of the Bidirectional Relationship between Diabetes and Periodontitis. Biomedicines, (2022), 10(1): 178.

[47] Simpson T C, Clarkson J E, Worthington H V, et al. Treatment of periodontitis for glycaemic control in people with diabetes mellitus. Cochrane Database of Systematic Reviews, (2022), 4(4):CD004714.

[48] Williams D W, Ho K, Lenon A, et al. Long‐term ligature‐induced periodontitis exacerbates development of bisphosphonate‐related osteonecrosis of the jaw in mice. Journal of Bone and Mineral Research, (2022), 37(7):1400-1410. 

[49] Suh J S, Kim S Y J, Lee S H, et al. Hyperlipidemia is necessary for the initiation and progression of atherosclerosis by severe periodontitis in mice. Molecular Medicine Reports, (2022), 26(2): 1-9.