Academic Journal of Medicine & Health Sciences, 2023, 4(12); doi: 10.25236/AJMHS.2023.041203.
Hao Zhao1, Xie Li2, Wu Yingying1
1Department of Oncology, The Second Naval Hospital of the Southern Theater Command of PLA, Sanya, China
2Department of Anesthesiology, Qilu Hospital of Shandong University Dezhou Hospital, Dezhou, China
To explore the effects and mechanism of TimosaponinA-III (Tim-AIII) on the proliferation and apoptosis of human liver cancer cells MHCC97H, an in vitro culture of MHCC97H cells was conducted. The logarithmic growth phase MHCC97H cells were divided into control group, Tim-AIII group, Tim-AIII + pc-DNA group, and Tim-AIII + pc-TGF-β1 group: the control group was MHCC97H cells, the Tim-AIII group was added with 40 nmol/L Tim-AIII, the Tim-AIII + pc-DNA group was transfected with blank control plasmid vector and added with 40 nmol/L Tim-AIII, and the Tim-AIII + pc-TGF-β1 group was transfected with TGF-β1 overexpression plasmid TGF-β1-OE and added with 40 nmol/L Tim-AIII. After culturing for 24 hours, the cloning formation experiment was used to detect the proliferation of each group of cells. Flow cytometry was used to detect the apoptosis rate of each group of cells. Western blot was used to detect the expression levels of TGF-β1 and ERK protein in each group of cells. The results showed that the numbers of clones in the control group, Tim-AIII group, Tim-AIII + pc-DNA group, and Tim-AIII + pc-TGF-β1 group were 221.69 ± 29.71, 32.18 ± 5.82, 31.92 ± 7.27, and 112.28 ± 23.42, respectively; the apoptosis rates were 13.60% ± 1.35%, 29.10% ± 2.71%, 30.00% ± 3.63%, and 21.70% ± 1.99%, respectively. Compared with the control group, the proliferation rate, clone number, and apoptosis rate of the Tim-AIII group and Tim-AIII + pc-DNA group were significantly different (P < 0.05); compared with the Tim-AIII group and Tim-AIII + pc-DNA group, the proliferation rate, clone number, and apoptosis rate of the Tim-AIII + pc-TGF-β1 group were significantly different (P < 0.05). Compared with the control group, the relative expression levels of TGF-β1 and ERK protein in the Tim-AIII group were significantly decreased (P < 0.05), while the relative expression levels of TGF-β1 and ERK protein in the pc-TGF-β1 group were significantly increased (P < 0.05); compared with the pc-TGF-β1 group, the relative expression levels of TGF-β1 and ERK protein in the Tim-AIII + pc-TGF-β1 group were significantly decreased (P < 0.05); compared with the Tim-AIII group, the relative expression levels of TGF-β1 and ERK protein in the Tim-AIII + pc-TGF-β1 group were significantly increased (P < 0.05). These results suggest that Tim-AIII can inhibit the proliferation of human liver cancer cells MHCC97H and promote apoptosis, which may be related to inhibiting the TGF-β1/ERK signaling pathway.
Liver cancer; TimosaponinA-III; Proliferation; Apoptosis; TGF-β1/ERK
Hao Zhao, Xie Li, Wu Yingying. The Effect and Mechanism of Timosaponina-III from Anemarrhena Asphodeloides on Proliferation and Apoptosis of Human Liver Cancer Cell Line MHCC97H. Academic Journal of Medicine & Health Sciences (2023) Vol. 4, Issue 12: 20-26. https://doi.org/10.25236/AJMHS.2023.041203.
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