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International Journal of Frontiers in Medicine, 2024, 6(5); doi: 10.25236/IJFM.2024.060517.

Aerobic Exercise Activates the Signaling Pathway of IGF-1/PI3K/Akt to Inhibit Skeletal Muscle Inflammation in Obese Mice

Author(s)

Hao Meili

Corresponding Author:
Hao Meili
Affiliation(s)

Physical Education Department, Luoyang Normal University, Luoyang, 471934, Henan, China

Abstract

This study aimed at discussing the effect of aerobic exercise on expression of insulin-like growth factor 1 (IGF-1) and its receptor Insulin like growth factor-1 receptor (IGF-1R) in skeletal muscle of obese mice, downstream signaling pathway of PI3K/Akt, skeletal muscle inflammation and atrophy. 35 healthy male C57BL/6 mice were randomly divided into normal control group (C, 10 mice) and high-fat diet group (25 mice). After 8 weeks, 20 obese mice were randomly divided into obesity control group (OS) and obesity exercise group (OE), 10 mice in each group. The OE group underwent treadmill aerobic exercise for 8 weeks. After training, the protein expression of IGF-1/IGF-1R, pPI3K/PI3K, pAkt/Akt, TNF-α, IL-1β, IL-10, Atrogin-1 and MuRF1 in skeletal muscle were measured by Western blotting. The cross-sectional area of skeletal muscle was observed and calculated by HE staining. The results showed that compared with C group, body weight in OS group was significantly increased, skeletal muscle IGF-1/IGF-1R, pPI3K/PI3K, pAkt/Akt and IL-10 protein expression were significantly downregulated, while TNF-α, IL-1β, Atrogin-1 and MuRF1 protein expression were significantly upregulated, the skeletal muscle cross-sectional area and gastrocnemius/body weight ratio were significantly decreased. Compared with OS group, body weight in OE group was significantly decreased, skeletal muscle IGF-1/IGF-1R, pPI3K/PI3K, pAkt/Akt and IL-10 protein expression were significantly upregulated, while TNF-α, IL-1β, Atrogin-1 and MuRF1 protein expression were significantly downregulated, the skeletal muscle cross-sectional area and gastrocnemius/body weight ratio were significantly increased. Conclusions are as follows, Aerobic exercise may via upregulate the expression of IGF-1 and its receptor IGF-1R protein in skeletal muscle of obese mice, activates the signaling pathway of PI3K/Akt, and inhibit inflammatory response. It shows that the signaling pathway of IGF-1/PI3K/Akt plays an important role in aerobic exercise inhibits the skeletal muscle inflammation of obese mice and improves skeletal muscle atrophy.

Keywords

aerobic exercise,obesity, IGF-1/PI3K/Akt signaling pathway, inflammation, skeletal muscle atrophy

Cite This Paper

Hao Meili. Aerobic Exercise Activates the Signaling Pathway of IGF-1/PI3K/Akt to Inhibit Skeletal Muscle Inflammation in Obese Mice. International Journal of Frontiers in Medicine (2024), Vol. 6, Issue 5: 121-127. https://doi.org/10.25236/IJFM.2024.060517.

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