The Frontiers of Society, Science and Technology, 2024, 6(12); doi: 10.25236/FSST.2024.061204.
Man Cheng1, Huijun Liu2, Tianhao Liu3
1Shandong Guanfu Holdings Group Co., Ltd., Jinan, Shandong, 250300, China
2Northeast Forestry University, Harbin, Heilongjiang, 150006, China
3Henan Finance University, Zhengzhou, Henan, 451464, China
In this study, the branches of apple dwarf rootstock variety T337 that had not yet germinated during the dormant period and the young branches of the virus-free mother plant with native M26, GM256 and M9 were used as the test materials, a variety of methods for establishing aseptic systems were designed, and the best ways and methods were selected. We found that the most suitable method for disinfecting explants is a mixture of 70% alcohol for 12 seconds, 0.1% mercuric chloride, and Tween for 7 minutes, followed by 10 washes with sterile water; The optimal subculture medium for T337 is MS+IBA 0.6 mg/L+6-BA 0.4 mg/L. MS+6-BA 0.7 mg/L+IBA 0.6 mg/L is suitable for M9 and GM256. The differentiation effect of M26 is good when MS+6-BA 0.9 mg/L+IBA 0.7 mg/L; 1/2 WPM+IBA 0.6 mg/L+NAA 0.02 mg/L has the best rooting effect; Domesticated substrate perlite: vermiculite: peat=1:1:3, temperature controlled at 23-28 ℃, humidity gradually decreased from high humidity, and light gradually increased from low light to adapt to natural environment. It is most suitable for seedling growth and is more conducive to production application and variety promotion.
Apple dwarf rootstock; Aseptic system; Proliferation and differentiation; Rooting and domestication
Man Cheng, Huijun Liu, Tianhao Liu. Research on the Optimization of Tissue Culture Technology Applied to the Production of Several Apple Dwarf Rootstocks. The Frontiers of Society, Science and Technology (2024), Vol. 6, Issue 12: 19-26. https://doi.org/10.25236/FSST.2024.061204.
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