Mengxing Lv, Jing Lv, Xihan Zhang, Liting Qiu, Jianzhou Ma, Yanan Ruan, Song Jin, Jiani Liu, Zebin Chen*
School of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan Province, China
This paper aims to optimize the rapid propagation system of strawberry tissue culture seedlings based on the intermittent submerged bioreactor (TIBs). The tissue culture plantlet of “Benihoppe” after primary culture was used as the explant and the TIBs system was used for rapid propagation, so as to study the effects of different generations, inoculation densities, intermittent immersion frequencies and immersion time on the proliferation of strawberry tissue culture seedlings. The results showed that the multiplication times of the third, fourth, fifth and sixth generations were higher, and multiplication rate of the fifth generation was the highest with 70.0 times. When the inoculation density was more than 4 strains / bottle, the pollution rate was 15.8%; when the inoculation density was less than 3 strains / bottle, there was no pollution. When the frequency of intermittent immersion was more than 5 min / 3 h, the pollution rate of tissue culture seedlings increased with the increase of intermittent immersion frequency. When the frequency of intermittent immersion reached 5 min / 4 h, the pollution rate was the highest, and the growth potential of tissue culture seedlings was also poor. The highest multiplication was 71.0 times after 25 minutes immersion every 1 hour. Therefore, good culture effect can be obtained by selecting the 5th generation of subculture plantlets, adopts the inoculation density of 2 plants / bottle and the intermittent immersion frequency of 10 min / 1 h, and uses the hormone combination of 3.0 mg / L 6 - BA + 0.01 mg / L NAA.
Strawberry, Immersion, Bioreactor, Tissue culture seedling, Rapid propagation
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