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The Frontiers of Society, Science and Technology, 2020, 2(11); doi: 10.25236/FSST.2020.021119.

Downregulating Lunar1 to “Notch-Down” the Progression of Human t-All

Author(s)

Qitong Luan

Corresponding Author:
Qitong Luan
Affiliation(s)

Gaston Day School

Abstract

T-ALL is one of the most common types of leukemia with a high lethality rate. The effective leukemia treatment CAR-T therapy is difficult to be applied on T-ALL. Previous studies has reported that the Notch-regulated lncRNA LUNAR1 upregulates IGF1R, which increases the cell proliferation in T-ALL cell lines. The silencing of LUNAR1 will downregulate the expression of IGF1 R and reduce T-ALL cell proliferation. This study investigates the effect of knocking down LUNAR1 using LUNAR1 siRNA on T-ALL treatment, in both in vitro and in vivo conditions. The experiments will use know human leukemia cell lines, a variety of pediatric primary thymus surgical samples and peripheral blood samples, murine cell lines, and Xenograft Murine Models. There are three most possible results: (1) Knockdown of LUNAR1 inhibit the T-ALL cell proliferation in both in vitro and in vivo cell lines; (2) Knockdown of LUNAR1 only inhibit the T-ALL cell proliferation in in vitro cell cultures; (3) Knockdown of LUNAR1 only inhibit the T-ALL cell proliferation in determined human and murine T-ALL cell lines. The result of this study will provide important information for the future clinical trial of LUNAR1 knockdown therapy. Future studies should focus on improving the in vivo delivery methods and finding more LUNAR1 drug inhibitors, as well as exploring the specific gene regulation mechanism of LUNAR1 in detail.

Keywords

T-all, Notch pathway, Lncrna, Lunar1, Igf1 r, Sirna therapy

Cite This Paper

Qitong Luan. Downregulating Lunar1 to “Notch-Down” the Progression of Human t-All. The Frontiers of Society, Science and Technology (2020) Vol. 2 Issue 11: 132-137. https://doi.org/10.25236/FSST.2020.021119.

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