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International Journal of Frontiers in Medicine, 2021, 3(1); doi: 10.25236/IJFM.2021.030101.

Exploring the potential of rosemary essential oil as an natural penetration enhancer for promoting transdermal absorption of crosslinked hyaluronic acid in-vitro


Jie Liu1, Haoming He2, Xiaoying Zhou1, *

Corresponding Author:
Xiaoying Zhou

1 School of Pharmacy, Changzhou University, Jiangsu, China, 213164
2 Changzhou Institute of Materia Medical, Changzhou, Jiangsu, China, 213164
*Corresponding author


Objectives: This investigation aimed to study the effect of rosemary essential oil on the transdermal absorption of crosslinked hyaluronic acid in vitro. Material and methods: The most suitable concentration of crosslinked hyaluronic acid was chosen and enhancing effects of rosemary essential oil on the permeation of crosslinked hyaluronic acid were evaluated. Results: The optimization of carbazole sulfate method by the 96-well plate was used to establish the standard curve of hyaluronic acid successfully. Crosslinked hyaluronic acid containing 5% (v/v) rosemary essential oil showed better skin penetration than blank control group in transdermal experiments. The combination of 5% (v/v) rosemary essential oil and 2%(v/v) azone as a permeability aid can achieve more effect than 5%(v/v) rosemary essential oil as a permeability aid. Conclusion: This study showed the enhancing effect of rosemary essential oil on crosslinked hyaluronic acid percutaneous absorption.


Rosemary essential oil, Crosslinked hyaluronic acid, Transdermal absorption, Natural penetration enhancers

Cite This Paper

Jie Liu, Haoming He, Xiaoying Zhou. Exploring the potential of rosemary essential oil as an natural penetration enhancer for promoting transdermal absorption of crosslinked hyaluronic acid in-vitro. International Journal of Frontiers in Medicine (2021), Vol. 3, Issue 1: 1-6. https://doi.org/10.25236/IJFM.2021.030101.


[1] Relleve L S, Gallardo A K R, Abad L V. Radiation crosslinking of carboxymethyl hyaluronic acid. Radiation Physics and Chemistry. 2018; 151: 211-216.
[2] Zhao N, Wang X, Qin L, et al. Effect of hyaluronic acid in bone formation and its applications in dentistry. Journal of Biomedical Materials Research Part A. 2016; 104 (6): 1560-1569.
[3] Shyam Vasvani, Pratik Kulkarni, Deepak Rawtani. Hyaluronic acid: A review on its biology, aspects of drug delivery, route of administrations and a special emphasis on its approved marketed products and recent clinical studies. International Journal of Biological Macromolecules; 2019.
[4] Cai B, S Derkvist K, Engqvist H, et al. A New Drug Release Method in Early Development of Transdermal Drug Delivery Systems. Pain Research and Treatment. 2012; 2012: 1-6.
[5] Herman A, Herman A P. Essential oils and their constituents as skin penetration enhancer for transdermal drug delivery: a review. Journal of Pharmacy and Pharmacology. 2015; 67 (4): 473-485.
[6] Carole E. Schanté, Zuber G , Herlin C , et al. Chemical modifications of hyaluronic acid for the synthesis of derivatives for a broad range of biomedical applications. Carbohydrate Polymers. 2011; 85 (3): 469-489.
[7] Song J M, Im J H, Kang J H, et al. A simple method for hyaluronic acid quantification in culture broth. Carbohydrate Polymers. 2009; 78 (3): 633-634.
[8] Cesaretti M, Luppi E, Maccari F, et al. A 96-well assay for uronic acid carbazole reaction. Carbohydrate Polymers. 2003; 54 (1): 59-61.
[9] Tomoyuki Kanmitsu. Action and mechanism of ingested hyaluronic acid. Gercosamine study. 2010; 6: 20-24.
[10] Dong Q , Zang H , Zang L , et al. Rapid determination of hyaluronic acid concentration in fermentation broth with near-infrared spectroscopy. Journal of Innovative Optical Health Sciences. 2014; 07 (06): 1450012.
[11] Ikeuchi M, Izumi M, Aso K, et al. Effects of intra-articular hyaluronic acid injection on immunohistochemical characterization of joint afferents in a rat model of knee osteoarthritis. European Journal of Pain. 2015; 19 (3): 334-340.
[12] Chou C L, Li H W, Lee S H, et al. Effect of Intra-articular Injection of Hyaluronic Acid in Rheumatoid Arthritis Patients with Knee Osteoarthritis. Journal of the Chinese Medical Association. 2008; 71 (8): 411-415.
[13] Patel V F, Liu F, Brown M B. Modeling the oral cavity: In vitro and in vivo evaluations of buccal drug delivery systems. Journal of Controlled Release. 2012; 161 (3): 746-756.
[14] Higo N. Recent trend of transdermal drug delivery system development. Yakugaku Zasshi Journal of the Pharmaceutical Society of Japan. 2010; 38 (32).
[15] Ahad A, Aqil M, Ali A. The application of anethole, menthone, and eugenol in transdermal penetration of valsartan: Enhancement and mechanistic investigation. Pharmaceutical Biology. 2016; 54 (6): 1042.
[16] Jun C, Qiu-Dong J, Ya-Ping C, et al. Natural Terpenes as Penetration Enhancers for Transdermal Drug Delivery. Molecules. 2016; 21 (12): 1709-1731.