Yiqing Li1,2, Jiayan Zheng1,2, Xiaoli Han1,2, Tingxin Wang1,2
1College of Quality and Technical Supervision, Hebei University, Baoding, China
2Institute of Food Safety and Standardization, Hebei University, Baoding, China
To explore the method of complete antigen synthesis of promazine hydrochloride. Based on the analysis of the molecular structure and immunogenicity of promazine hydrochloride, complete antigen was synthesized by mixed anhydride method,under the condition of excess acid, the diazotization reaction of nitrite and p-aminobenzoic acid produces diazosalt, diazonium salt reacts with promazine hydrochloride; Triethylamine and isobutyl chloroformate form a mixed anhydride, promazine hydrochloride complete antigen was synthesized by amino ligation of mixed anhydride and carrier protein bovine serum albumin (BSA), complete antigen synthesis was identified by ultraviolet spectroscopy, the characteristic absorption peaks of promazine hydrochloride, bovine serum albumin and synthetic promazine hydrochloride complete antigen were analyzed. The peak wavelength of the synthesized complete antigen, promazine hydrochloride and bovine serum albumin is different, the complete antigen synthesis of promazine hydrochloride was proved successful. The results of this study provided a basis for the rapid detection of promazine hydrochloride residues in animal-derived food by immunoassay.
Promazine hydrochloride, Complete antigen synthesis, Mixed anhydride method, Ultraviolet spectroscopic method, Veterinary drug residue
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