International Journal of Frontiers in Medicine, 2021, 3(3); doi: 10.25236/IJFM.2021.030309.
Yiqing Li1,2, Jiayan Zheng1,2, Xiaoli Han1,2, Tingxin Wang1,2
1College of Quality and Technical Supervision, Hebei University, Baoding, China
2Institute of Food Safety and Standardization, Hebei University, Baoding, China
To explore the method of complete antigen synthesis of promazine hydrochloride. Based on the analysis of the molecular structure and immunogenicity of promazine hydrochloride, complete antigen was synthesized by mixed anhydride method,under the condition of excess acid, the diazotization reaction of nitrite and p-aminobenzoic acid produces diazosalt, diazonium salt reacts with promazine hydrochloride; Triethylamine and isobutyl chloroformate form a mixed anhydride, promazine hydrochloride complete antigen was synthesized by amino ligation of mixed anhydride and carrier protein bovine serum albumin (BSA), complete antigen synthesis was identified by ultraviolet spectroscopy, the characteristic absorption peaks of promazine hydrochloride, bovine serum albumin and synthetic promazine hydrochloride complete antigen were analyzed. The peak wavelength of the synthesized complete antigen, promazine hydrochloride and bovine serum albumin is different, the complete antigen synthesis of promazine hydrochloride was proved successful. The results of this study provided a basis for the rapid detection of promazine hydrochloride residues in animal-derived food by immunoassay.
Promazine hydrochloride, Complete antigen synthesis, Mixed anhydride method, Ultraviolet spectroscopic method, Veterinary drug residue
Yiqing Li, Jiayan Zheng, Xiaoli Han, Tingxin Wang. Study on Complete Antigen Synthesis of Promazine Hydrochloride. International Journal of Frontiers in Medicine (2021), Vol. 3, Issue 3: 47-54. https://doi.org/10.25236/IJFM.2021.030309.
[1] No.235 Announcement of the Ministry of Agriculture of the People's Republic of China.Maximum Veterinary Drug Residues in Animal Foods, 2002.
[2] FENG Z W. Veterinary drugs and animal food safety [J]. Chinese Journal of Veterinary Medicine, 2004, 38(9):1-5.
[3] Establishment of a HPLC method for the determination of hexabenzylhexazazoisovertane [D]. Beijing Institute of Technology, 2015.
[4] K.Kawashima, R.dixon, S.Spector. Development of radioimmunoassay for chlorpromazine [J]. Eur.J.Pharmacol, 1975, 32: 195-202.
[5] Singh,KV,Kaur J,Varshney GC,et al. Synthesis and characterization of hapten-protein conjugates for antibodyproduction against small molecules[J]. Bioconjugate Chemistry, 2004, 15(1): 168-173.
[6] Wang YA, Wang XK, Niu LL, et al.Journal of Food Science and Technology, 2016, 37(23): 367-376.
[7] WANG JL, SHEN QR, YU Z, et al.Synthesis and characterization of phenobarbital artificial antigen [J]. Journal of NanChang University, 2015, 39(1):76-82.
[8] Bai J. Study on the synthesis of activated orange KN5R [D]. Donghua University, 2013.
[9] JIANG XJ. Determination of fatty acid value in rice by infrared spectroscopy [D]. Zhejiang University, 2018.
[10] QU J. Synthesis of azobenzene photochromic pigments [D]. Xi'an University of Technology, 2011.
[11] Guang TL. Research on the solution behavior of single stranded telomere DNA [D]. University of Science and Technology of China, 2018.
[12] Li RQ, Quan CH, Zhang LY, et al.Progress in artificial antigen synthesis [J]. Chinese Journal of Biological Engineering, 2018, 38(12):65-65.
[13] LIU JJ .Development of an enzyme-linked immunosorbent assay (ELISA) for chlorpromazine [D]. Chinese Academy of Agricultural Sciences, 2009