Academic Journal of Medicine & Health Sciences, 2021, 2(1); doi: 10.25236/AJMHS.2021.020108.
Yueqi Xu1, Yifeng Gong2, Yunfei Ji3, Hao Zhang4, Kaiyu Zhou5, Miaomiao Zhu6
1University of California-Santa Barbara, CA, USA
2Wuhan University, Wuhan, Hubei, China
3Capital Medical University, Beijing, China
4University of California Davis, CA, USA
5Qingdao No.2 High School, Qingdao, Shandong, China
6Wuxi Big Bridge Academy, Wuxi, Jiangsu, China
These authors contributed equally to this work
From the adipose tissue which credits as the biggest endocrine organs and significant tissue for homeostasis, people can derive a sort of mesenchymal stem cells (MSC) which is also learned as adipose tissue-derived stem cells (AD-MSC). This kind of stem cells is easier and safer for scientists to access and still have similar renewal ability and multipotent differentiation profile compared to those stem cells in bone marrow [1]. In this study, we investigate the possibility of adipose tissue-derived cells to differentiate into insulin producing cells. We cultured, characterized, and confirmed that AD-MSC can be successfully differentiated into pancreatic cell lineage which is insulin producing. Following a three-step protocol, we finally achieve insulin producing cells from AD-MSC. Through qCR analysis, marker genes of Nkx6.1, Pdx1, Ngn3, Neurod1, insulin, and glucagon are in presence in the final stage of cells. For further confirmation, we employed immunofluorescence staining on Pdx1 and insulin. The outcome shows the ultimate cells can be stained by DTZ with brown coloring. Furthermore, after exposing the cells to different levels of glucose, we can see the total rise of insulin and C peptide which proves that the cells have the ability to react to the glucose stimulation. The success of the differentiation of AD-MSC to insulin producing cells probably open the gate to new kind of applicable, convenient, and undisputed treatment for diabetes.
adipose tissue-derived Stem cells, insulin-producing cells, diabetes
Yueqi Xu, Yifeng Gong, Yunfei Ji, Hao Zhang, Kaiyu Zhou, Miaomiao Zhu. Differentiation of Adipose Tissue-derived Stem Cells into Insulin-Producing Cells for Improving the Diabetes Therapy. Academic Journal of Medicine & Health Sciences (2021) Vol. 2, Issue 1: 42-48. https://doi.org/10.25236/AJMHS.2021.020108.
[1] Baer, Patrick. (2012). Adipose Derived Mesenchymal Stem Cells.
[2] Graf T. Differentiation plasticity of hematopoietic cells[J]. Blood, 2002, 99(9):3089-101.
[3] Watt F M and Hogan B L. Out of Eden: stem cells and their niches[J]. Science, 2000, 287(5457):1427-30.
[4] Kern S, Eichler H, Stoeve J, et al. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue[J]. Stem Cells, 2006, 24(5):1294-301.
[5] Faustini M, Bucco M, Chlapanidas T, et al. Nonexpanded mesenchymal stem cells for regenerative medicine: yield in stromal vascular fraction from adipose tissues[J]. Tissue Eng Part C Methods, 2010, 16(6):1515-21.
[6] Zuk P A, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies[J]. Tissue Eng, 2001, 7(2):211-28.
[7] Ambasudhan R, Talantova M, Coleman R, et al. Direct reprogramming of adult human fibroblasts to functional neurons under defined conditions[J]. Cell Stem Cell, 2011, 9(2):113-8.
[8] Kim J, Lengner C J, Kirak O, et al. Reprogramming of postnatal neurons into induced pluripotent stem cells by defined factors[J]. Stem Cells, 2011, 29(6):992-1000.
[9] Karnieli O, Izhar-Prato Y, Bulvik S, et al. Generation of insulin-producing cells from human bone marrow mesenchymal stem cells by genetic manipulation[J]. Stem Cells, 2007, 25(11):2837-44.
[10] Lu Y, Wang Z and Zhu M. Human bone marrow mesenchymal stem cells transfected with human insulin genes can secrete insulin stably[J]. Ann Clin Lab Sci, 2006, 36(2):127-36.
[11] Cong Ran Li, Xue Fu You, Jian Dong Jiang. 2005. Animal models of diabetes mellitus and research progress. Chinese Journal of Comparative Medicine, 15:59-60
[12] Cao J.Y, et al. (2020) China Cardiovascular Health and Disease Report
[13] Yu W.H..In vitro differentiation of rabbit bone marrow mesenchymal stem cells into pancreatic islet cells[D]. Henan:Henan Agricultural University, 2016.
[14] Govindasamy V., Ronald V.S., Abdullah A.N., Ganesan Nathan K.R., Ab Aziz Z.A.C., Abdullah M., Musa S., Abu Kasim N.H., Bhonde R.R. (2011) Differentiation of Dental Pulp Stem Cells into Islet-like Aggregates. J DENT RES 90(5):646-52.
[15] Mazini L., Rochette L., Amine M., Malka1G. (2019) Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs). Int J Mol Sci 20(10): 2523.