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Academic Journal of Medicine & Health Sciences, 2021, 2(1); doi: 10.25236/AJMHS.2021.020109.

Effect of Eurotium cristatum fermentation on the α-glucosidase inhibitory activity of mulberry leaves alkaloids

Author(s)

Yangcheng Gao1, Rong Chen2, Yinqi Zhao3

Corresponding Author:
Yangcheng Gao
Affiliation(s)

1Michigan State University, ML, USA

2Providence country day school, RI,USA

3International department of Beijing No.35 High School, Beijing, China

These authors contributed equally to this work

Abstract

Eurotium cristatum CY-1 was used for the solid-stated fermentation of mulberry leaves (ML) with the purpose of enhancing the alkaloids content and α-glucosidase inhibitory activity of ML through microbial fermentation. The results indicated that Eurotium cristatum CY-1 grown well when cultivated with MLs as substrate. The maximum cell dry weight of 393.32 mg was reached on the 10 d of fermentation. The alkaloids content of ML was increased with the fermentation time, and the maximum value of 8.711 mg/g was obtained at 8 d of fermentation, which was increased by171.96% compared to that extracted from unfermented ML. The α-glucosidase inhibitory efficiency was also improved along with the fermentation time. The IC50 values of alkaloid extracted from 8d-fermented mulberry leaves was 3.94 μg/mL, but 6.82 μg/mL of the alkaloid extracted from the unfermented ML. Therefore, the fermentation of Eurotium cristatum could benefit for enhancing the alkaloid content and α-glucosidase inhibitory efficiency of ML. It would provide a valuable strategy for obtain safe and low-cost α-glucosidase inhibitors from natural herbal medicines.

Keywords

Mulberry leaf, Eurotium cristatum, Alkaloids, α-glucosidase inhibitory, Diabetes

Cite This Paper

Yangcheng Gao, Rong Chen, Yinqi Zhao. Effect of Eurotium cristatum fermentation on the α-glucosidase inhibitory activity of mulberry leaves alkaloids. Academic Journal of Medicine & Health Sciences (2021) Vol. 2, Issue 1: 49-54. https://doi.org/10.25236/AJMHS.2021.020109.

References

[1] Benmelouka, A. Y., Abdelaal, A., Mohamed, A. S. E., et al. Association between sarcoidosis and diabetes mellitus: a systematic review and meta-analysis. Expert Rev Respir Med. 2021, 12: 1-7. 

[2] Kaur, N., Kumar, V., Nayak, S. K., et al. Alpha-amylase as molecular target for treatment of diabetes mellitus: A comprehensive review. Chemical Biology & Drug Design, 2021, 00, 1– 22.

[3] Thomas, R.L., Halim, S., Gurudas, S., et al. IDF Diabetes Atlas: A review of studies utilising retinal photography on the global prevalence of diabetes related retinopathy between 2015 and 2018, Diabetes Research and Clinical Practice, 2019. 157: 107840

[4] Shi, J.Y., Zhang, R., Liang, F.Q., et al. Screening of α-glucosidase inhibitors from cereal phytochemicals and underlying molecular mechanism. Food Science, 2021, 42(5): 9-16.

[5] Lin, A. H., Lee, B. H., Chang, W. J. Small intestine mucosal α-glucosidase: A missing feature of in vitro starch digestibility. Food Hydrocoll, 2016, 53 (FEB.): 163-171.

[6] Shah, M, A,, Khalil, R,, Ulhaq, Z., et al. α-Glucosidase inhibitory effect of rhinacanthins-rich extract from Rhinacanthus nasutus leaf and synergistic effect in combination with acarbose. Journal of Functional Foods, 2017, 36: 325-331. 

[6] Zhi L.C., Zhang L.Y., Liang X.Y., et al. Research progress on the inhibitory effect of natural active ingredients on α-glucosidase. Journal of Food Safety and Quality. 2021, 12(6): 2276-2282.

[7] Cao, F.H., Zhang, J.H., Wang, Y.P. Optimization of activated carbon decolorization process of mulberry leaf alkaloids and its α-glucosidase inhibitory activity. Science and Technology of Food Industry, 2020, 41(13): 162-167.

[8] Kwon, O.C., Ju, W.T., Kim, H.B., et al. Characterization of DNJ production for large-scale fermentation of mulberry leaf. Int. J. Indust. Entomol. 2017, 35(2): 111-117.

[9] Fan, H.B., Huang C.Y., Xu G.R., et al. The study of indirect determination of biomass in solid-state fermentation of Monascus by the measurement of glucosamine content. Microbiology China, 2014, 41(9): 1909-1916.

[10] Majouli, K., Besbes, H. M., Hamdi, A., et al. Antioxidant activity and α-glucosidase inhibition by essential oils from Hertia cheirifolia (L.). Industrial Crops and Products, 2016, 82: 23-28.

[11] Şöhretoğlu, D., Sari, S., Özel, A., et al. α-Glucosidase inhibitory effect of Potentilla astracanica and some isoflavones: Inhibition kinetics and mechanistic insights through in vitro and in silico studies. International Journal of Biological Macromolecules, 2017, 105: 1062-1070.

[12] Du, H.; Wang, Q.; Yang, X. Fu Brick Tea alleviates chronic kidney disease of rats with high fat diet consumption through attenuating insulin resistance in skeletal muscle. Agric. Food Chem. 2019, (67):2839–2847.

[1] Gu, Q.; Duan, G.; Yu, X. Bioconversion of flavonoid glycosides from Hippophae rhamnoides leaves into flavonoid aglycones by Eurotium amstelodami. Microorganisms 2019, (7):122.

[13] Xiao, Y.; Sun, M.; Zhang, Q.; et al. Effects of Cordyceps militaris (L.) Fr. fermentation on the nutritional, physicochemical, functional properties and angiotensin I converting enzyme inhibitory activity of red bean (Phaseolus angularis [Willd.] WFWight.) flour. Food Science and Technology, 2018, (55):1244–1255.

[14] Zou, M., Guo, X., Huang, Y., et al. Improvement of the quality of Ginkgo biloba leaves fermented by Eurotium cristatum as high value-added feed.Processes 2019, (7): 627.

[15] Zhu, M.Z.; Li, N.; Zhou, F.; Ouyang, J.; Lu, D.M.; Xu, W.; Li, J.; Lin, H.; Zhang, Z.; Xiao, J.B.; et al. Microbial bioconversion of the chemical components in dark tea. Food Chem. 2020, (312): 126043.

[16] Xiao, Y., Huang, Y., Chen, Y., et al. Effects of Solid-State Fermentation with Eurotium cristatum YL-1 on the Nutritional Value, Total Phenolics, Isoflavones, Antioxidant Activity, and Volatile Organic Compounds of Black Soybeans.Agronomy 2021, (11): 1029.

[17] Du, J., Wang, Q.Q., Wang, Y.S., et al. Effect on bioactive components and antioxidant activity of Pueraria lobata fermented by Eurotium cristatum. Science and Technology of Food Industry. 2021, 42(1): 121-126

[18] Chan, L.Y., Takahashi, M., Lim, P.J., et al. Eurotium Cristatum fermented Okara as a potential food ingredient to combat Diabetes. Scientific Reports. 2019, (9):17536