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Academic Journal of Medicine & Health Sciences, 2021, 2(1); doi: 10.25236/AJMHS.2021.020111.

Enhance the flavonoids content and α-glucosidase inhibitor efficiency of mulberry leaves by fermented with Ganoderma lucidum


Yihao Luo1, Yuzhao Zhuo2, Meng Cai3, Mingqian Xu4, Zikai Zhou5

Corresponding Author:
Yihao Luo

1Guangdong Technion-Israel Institute of Technology, Shantou, Guangdong, China

2Guangdong Technion-Israel Institute of Technology, Shantou, Guangdong, China

3Henan University of Technology, Zhengzhou, Henan, China

4Weifang NO.7 Middle School, Weifang, Shandong, China

5Ningbo Hanvos Kent School, Ningbo, Zhejiang, China

These authors contributed equally to this work


Diabetes is a kind of chronic disease that seriously threatens human health. α-glucosidase inhibitors are an effective way to inhibit the increase of postprandial blood glucose. Mulberry leaf flavonoids can be used as highly effective α-glucosidase inhibitors, but usually in low concentrations. Microbial fermentation is an effective method to increase the content of flavonoids in plants. In this paper, the fermentation of mulberry leaves with Ganoderma lucidum was conducted to investigate the cell growth of microbial, the content and α-glucosidase inhibitory efficiency of flavonoids in mulberry leaves. The results indicated that mulberry leaves can satisfy the nutritional needs of G. lucidum, and the maximum cell dry weight of 393.32 mg was obtained after a 10 d of fermentation. The flavonoid content in mulberry leaves was increased significantly with the fermentation time. Compared to unfermented mulberry leaves, the flavonoid content in mulberry leaves fermented for 8 d increased by 56.5%. The inhibitory efficiency of the fermented flavonoids was also significantly improved. The IC50 of α-glucosidase of flavonoids extracted from 8 d fermented mulberry leaves and unfermented mulberry leaves were 0.041 mg/mL and 0.01 mg/mL, respectively. This article will provide a reference for the enrichment of functional active ingredients in plants through microbial fermentation technology.


Mulberry leaves, α-glucosidase inhibitor, Ganoderma lucidum, Flavonoids, Microbial fermentation technology

Cite This Paper

Yihao Luo, Yuzhao Zhuo, Meng Cai, Mingqian Xu, Zikai Zhou. Enhance the flavonoids content and α-glucosidase inhibitor efficiency of mulberry leaves by fermented with Ganoderma lucidum. Academic Journal of Medicine & Health Sciences (2021) Vol. 2, Issue 1: 62-68. https://doi.org/10.25236/AJMHS.2021.020111.


[1] Cao, J., Zhang, X.Y., Zhou, B., Lu, Y. (2019). Study on the extraction technology of total flavones from mulberry leaves. Food and Fermentation Sciences & Technology, 55(4), 66-70+74. 

[2] Cao, Y., Jiang, W., Bai, H., Li, J. (2021). Study on active components of mulberry leaf for the prevention and treatment of cardiovascular complications of diabetes. Journal of Functional Foods, 83, 104549.

[3] Chaudhury, A., Duvoor, C., Reddy Dendi, V. S., Kraleti, S. (2017). Clinical Review of Antidiabetic Drugs: Implications for Type 2 Diabetes Mellitus Management. Front Endocrinol (Lausanne), 8, 6. 

[4] Chunli, S., Man, L., Jianyu, S., Jinli, Z. (2019). Nutrient changes in solid-state fermented okara with Ganoderma lucidum. Food and Fermentation Industries, 45(12), 114-120. 

[5] Fan, H.B., Huang, C.Y., Xu, G.R, et al (2014). The study of indirect determination of biomass in solid-state fermentation of Monascus by the measurement of glucosamine content. Microbiology China, 41, 1909-1916. 

[6] Fan, L., Wang, Y.L., Li, T. (2016). Review on screening methods for alpha-glucosidase inhibitors from natural resources. Nat Prod Res Dev, 28, 313-321+306. 

[7] Hansawasdi, C., Kawabata, J. (2006). α-Glucosidase inhibitory effect of mulberry (Morus alba) leaves on Caco-2. Fitoterapia, 77(7), 568-573. 

[8] He, L.Y., Lin, Z.C., Lu, J.D., Xiong, G.L. (2021). Detoxification and sustained effects of Tripterygium wilfordii based on Ganoderma lucidum bi-directional solid fermentation. Journal of Beijing University of Chemical Technology (Natural Science), 48(4), 48-56. 

[9] Inzucchi, S. E., Bergenstal, R. M., Buse, J. B., Diamant, M. (2015). Management of hyperglycemia in type 2 diabetes, 2015: a patient-centered approach: update to a position statement of the american diabetes association and the european association for the study of diabetes. Diabetes Care, 38(1), 140. 

[10] Ji, T, Shu, S., Sheng, G., Ouyang, Z. (2015). Research progress on bioactive component groups and their action mechanisms of Mori Folium for prevention and treatment of diabetes. Chinese Traditional and Herbal Drugs, 46(5), 778-784. 

[11] Li, Q., Wang, C., Liu, F., Hu, T.. (2020). Mulberry leaf polyphenols attenuated postprandial glucose absorption via inhibition of disaccharidases activity and glucose transport in Caco-2 cells. Food Funct, 11(2), 1835-1844. 

[12] Liu, Z.Z., Liu, Q.H., Liu, Z., Tang, J.W.. (2021). Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats. BMC Complementary Medicine and Therapies, 21(1), 172. 

[13] Majouli, K., Besbes Hlila, M., Hamdi, A., Flamini, G. (2016). Antioxidant activity and α-glucosidase inhibition by essential oils from Hertia cheirifolia (L.). Industrial Crops and Products, 82, 23-28. 

[14] Meng, Q., Qi, X., Fu, Y., Chen, Q.. (2020). Flavonoids extracted from mulberry (Morus alba L.) leaf improve skeletal muscle mitochondrial function by activating AMPK in type 2 diabetes. Journal of Ethnopharmacology, 248, 112326.

[15] Li, Q.Y., Lin, L.B., Yang, X.J., Tan, C.Y., Deng, X.Y. (2021). Research status of microbial fermentation of Chinese herbal medicine. Microbiology China, 48(6), 2232-2244. 

[16] Shen, C.L., Sha, J.Y., Li, M., Zhang, J.L. (2019). Study on the change of antioxidant properties of okara solid-fermented by Ganoderma lucidum. Food Research And Development, 40(24), 60-64. 

[17] Şöhretoğlu, D., Sari, S., Özel, A., & Barut, B. (2017). α-Glucosidase inhibitory effect of Potentilla astracanica and some isoflavones: Inhibition kinetics and mechanistic insights through in vitro and in silico studies. International Journal of Biological Macromolecules, 105, 1062-1070. 

[18] Tan, Z.X., Xu, L.J., & Chen, S.B. (2018). Rrsearch progress of α-glucosidase inhibitors derived from plant sources. Central South Pharmacy, 16(7), 982-987. 

[19] Thomas, R. L., Halim, S., Gurudas, S., Sivaprasad, S. (2019). IDF Diabetes Atlas: A review of studies utilising retinal photography on the global prevalence of diabetes related retinopathy between 2015 and 2018. Diabetes Research and Clinical Practice, 157, 107840. 

[20] Wu, J., Kaewnarin, K., Nie, X., Li, Q. (2021). Biological activities of a polysaccharide from the coculture of Ganoderma lucidum and Flammulina velutipes mycelia in submerged fermentation. Process Biochemistry, 109, 10-18.

[21] Xiong, Y.H., Li, J., Huang, S. (2011). Content determination of total polysaccharides in Panax notoginseng. Asia-Pacific Traditional Medicine, 7(7), 7-9. 

[22] Wu, Y.X., Wu, L.P., Park, E., Kim, T. (2020). Changes in main functional substances and biological activities of okara fermented with medicinal and edible fungi. Food and Fermentation Industries, 46(15), 100-106. 

[23] Zhang, L., Chen, Q., Li, L., Kwong, J. S. (2016). Alpha-glucosidase inhibitors and hepatotoxicity in type 2 diabetes: a systematic review and meta-analysis. Sci Rep, 6, 32649.

[24] Zhang, Q.M., Xu, Y.Y., Chen, M.H., Sun, Z.W., Su, H. (2019). Study on the changes of main active substances in Ganoderma lucidum solid fermentation ganmao qingre granule drug residue. Military Medical Journal of Southeast China, 21(6), 616-620. 

[25] Zhi, L.C., Zhang, L.Y., & Liang, X.Y., et al. (2021). Research progress on the inhibitory effect of natural active ingredients on α-glucosidase. Journal of Food Safety and Quality, 12(6), 2276-2282. 

[26] Zhu, Y.P,. Cheng,Y.Q., Liu, H.J., Li, L.T., Yamaki, K. (2008). α-Glucosidase inhibitory activity of okara fermented with various microorganisms. Journal of the Chinese Cereals and Oils Association, 23(4), 70-75.