Guorong Wang1,2, Yukun Xue3, Jie Lv4
1Clinical Laboratory, Yinan County People's Hospital, Linyi 276000, Shandong, China
2Medical Laboratory Specialty, Binzhou Medical College, Binzhou 256600, Shandong, China
3Clinical Laboratory, Pingyi County Traditional Chinese Medicine Hospital, Linyi 273300, Shandong, China
4Clinical Laboratory, People's, Hospitai of Rizhao, Rizhao 276800, Shandong, China
Nano silver is a single silver particle between certain chemical means and physical means. Its particle size is between 1-100nm. Nano silver is easy to enter the body of pathogens, so as to control infection and promote wound healing. Therefore, nano silver medical devices such as nano silver and nano silver dressing are widely used in clinical medicine. At the same time, nano silver materials also have certain biological characteristics, which may lead to the biological effects of nano silver materials, but so far, no complete conclusion has been reached. In recent years, a large number of cytotoxicity experiments have been carried out on silver nanoparticles in vitro. Therefore, this paper studies and discusses the in vitro cytotoxicity test of nano silver and its pharmaceutical products through the in vitro cytotoxicity test of nano materials, and provides the basis and support for the in vitro cytotoxicity test of nano materials by using the key project indicators in MTT test and LDH test. The selection of MTT test and LDH test is due to the characteristics of MTT method and the cytotoxicity of silver nanoparticles in vitro. The combination of MTT test and LDH test can evaluate the cytotoxicity of nano materials more comprehensively and study the in vitro toxicity of silver nanoparticles to mammalian cells. According to the experimental results in this paper, we can infer the toxicity mechanism of nano silver materials, which has a certain idea for the application of nano silver and nano silver materials. Through the discovery of in vitro cytotoxicity mechanism of nano silver medical devices, nano silver and its medical devices can be used more comprehensively and safely.
Nano Silver, Cytotoxicity in Vitro, MTT Test, LDH Test
Guorong Wang, Yukun Xue, Jie Lv. In Vitro Cytotoxicity Test of Nano Silver Medical Devices. International Journal of Frontiers in Medicine (2021), Vol. 3, Issue 4: 42-50. https://doi.org/10.25236/IJFM.2021.030408.
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