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International Journal of Frontiers in Medicine, 2021, 3(4); doi: 10.25236/IJFM.2021.030408.

In Vitro Cytotoxicity Test of Nano Silver Medical Devices


Guorong Wang1,2, Yukun Xue3, Jie Lv4

Corresponding Author:
Yukun Xue

1Clinical Laboratory, Yinan County People's Hospital, Linyi 276000, Shandong, China

2Medical Laboratory Specialty, Binzhou Medical College, Binzhou 256600, Shandong, China

3Clinical Laboratory, Pingyi County Traditional Chinese Medicine Hospital, Linyi 273300, Shandong, China

4Clinical Laboratory, People's, Hospitai of Rizhao, Rizhao 276800, Shandong, China


Nano silver is a single silver particle between certain chemical means and physical means. Its particle size is between 1-100nm. Nano silver is easy to enter the body of pathogens, so as to control infection and promote wound healing. Therefore, nano silver medical devices such as nano silver and nano silver dressing are widely used in clinical medicine. At the same time, nano silver materials also have certain biological characteristics, which may lead to the biological effects of nano silver materials, but so far, no complete conclusion has been reached. In recent years, a large number of cytotoxicity experiments have been carried out on silver nanoparticles in vitro. Therefore, this paper studies and discusses the in vitro cytotoxicity test of nano silver and its pharmaceutical products through the in vitro cytotoxicity test of nano materials, and provides the basis and support for the in vitro cytotoxicity test of nano materials by using the key project indicators in MTT test and LDH test. The selection of MTT test and LDH test is due to the characteristics of MTT method and the cytotoxicity of silver nanoparticles in vitro. The combination of MTT test and LDH test can evaluate the cytotoxicity of nano materials more comprehensively and study the in vitro toxicity of silver nanoparticles to mammalian cells. According to the experimental results in this paper, we can infer the toxicity mechanism of nano silver materials, which has a certain idea for the application of nano silver and nano silver materials. Through the discovery of in vitro cytotoxicity mechanism of nano silver medical devices, nano silver and its medical devices can be used more comprehensively and safely.


Nano Silver, Cytotoxicity in Vitro, MTT Test, LDH Test

Cite This Paper

Guorong Wang, Yukun Xue, Jie Lv. In Vitro Cytotoxicity Test of Nano Silver Medical Devices. International Journal of Frontiers in Medicine (2021), Vol. 3, Issue 4: 42-50. https://doi.org/10.25236/IJFM.2021.030408.


[1] ZHENG SU-fen, LIU WEN-ting,LI Ju-mei, Effect of Nano Silver Antibacterial Combined with New Dressing on stage Ⅱ-Ⅳ Pressure Sores in Elderly Patients with Mental Distress, Practial Clinical Medicine,2020,Vol21,No2: 74

[2] Wei L, Tang J, Zhang Z, et al. Investigation of the cytotoxicity mechanism of silver nanoparticles in vitro[J]. Biomedical Materials, 2016, 5(4):044103.

[3] Yijuan Song, Rongfa Guan, Fei Lyu, In vitro cytotoxicity of silver nanoparticles and zinc oxide nanoparticles to human epithelial colorectal adenocarcinoma (Caco-2) cells[J]. Mutation Research, 2015, 769:113-118.

[4] Tomankova K , Horakova J , Harvanova M , et al. Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro[J]. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2015, 82(6):106-115.

[5] Jiang X , Wu Y , Gray P , et al. Influence of gastrointestinal environment on free radical generation of silver nanoparticles and implications for their cytotoxicity[J]. Nanoimpact, 2018, 10:144-152.

[6] Anna, Barbasz, K. Barbara, and O. Magdalena. "How the surface properties affect the nanocytotoxicity of silver? Study of the influence of three types of nanosilver on two wheat varieties." Acta Physiologiae Plantarum 40.2(2018):31.

[7] Liu L , Wu Y , Xu C , et al. Synthesis, Characterization of Nano- β -Tricalcium Phosphate and the Inhibition on Hepatocellular Carcinoma Cells[J]. Journal of Nanomaterials, 2018, 2018(2):1-7.

[8] Liu, Chenghu, et al. "Strategy on biological evaluation forbiodegradable/absorbable materials andmedical devices." Bio-medical materials and engineering 29.3(2018):269-278.

[9] Xie, Linna, et al. "Cellular glucose metabolism is essential for the reduction of cell-impermeable water-soluble tetrazolium (WST) dyes." International journal of biological ences 14.11(2018):1535-1544.

[10] Ishiyama M , Miyazono Y , Shiga M , et al. Notes Benzothiazole-Containing Tetrazolium Salts That Produce Water-Soluble Formazan Dyes Absorbing at a Long Wavelength upon NADH Reduction[J]. Analytical Sciences, 2016, 12(3):515-519.

[11] Mark D. Kovacs, Douglas H. shearor, Paul g. Thacker, et al. Metrix matrix: a cloud based system for tracking non – relative value unit value added work metrics [J]. Journal of the American College of Radiology, JACR, 2018, 15 (3 pt a): 415

[12] Yang M, Gu L, Yang B, et al. Antifouling composites with self-adaptive controlled release based on an active compound intercalated into layered double hydroxides [J]. Applied Surface ence, 2017, 426(dec.31):185-193.

[13] Wang W, Kan Y, Pan H, et al. Phosphorylated cellulose Applied for the Exfoliation of LDH: An Advanced Reinforcement for Polyvinyl Alcohol [J]. Composites Part A: Applied ence and Manufacturing, 2016, 94(Complete):170-177.

[14] Myrick M L , Greer A E , Nieuwland A , et al. Fine-Structure Measurements of Oxygen A Band Absorbance for Estimating the Thermodynamic Average Temperature of the Earth\"s Atmosphere. An Experiment in Physical and Environmental Chemistry [J]. Journal of Chemical Education, 2016, 83(2):263.

[15] Terzi S, zgür, A, Erdivanli, et al. Diagnostic value of the wideband acoustic absorbance test in middle-ear effusion [J]. Journal of Laryngology & Otology, 2015, 129(11):1078-1084.