Objective: To observe the effects of sodium new houttuyfonate on proliferation, apoptosis and migration of human epithelial ovarian cancer A2780 cells, and to investigate the inhibition of invasion and migration of human epithelial ovarian cancer A2780 cells by regulating the expression of apoptosis-related molecules. Methods: blank group and different dose groups (100, 200, 300,400 and 500 μg·ml-1of sodium new houttuyfonate) were treated with human ovarian cancer A2780 cells for 48 h, respectively. MTT assay was used to detect cell proliferation and activity, and flow cytometry was used to detect cell proliferation inhibition rate and apoptosis rate. The cell migration was observed and the healing rate and migration rate of scratch were calculated. The mRNA expression of apoptosis-related molecules Bcl-2, Bax, VEGF and NF-κBp65 was detected by real-time PCR. Results: MTT and flow cytometry results showed that compared with blank group, the cell inhibition rate of sodium neohouttuynium was significantly increased (P<0.01), and the total cell apoptosis rate was increased (P<0.05); The results of scratch test showed that compared with blank group, the migration ability of new houttuynia sodium group was weakened, and the number of cell migration was significantly reduced. PCR results showed that compared with the blank group, the mRNA expression of VEGF, Bcl-2 and NF-κBp65 in the group of neohouttuynia sodium decreased, and the expression of Bax increased. Conclusion: sodium new houttuyfonate can inhibit proliferation and promote apoptosis of human ovarian cancer A2780 cells, and may inhibit the migration of A2780 cells by regulating the expression of apoptosis-related molecules.

Linsong Yang, Luyao Wang. Effects of sodium new houttuyfonate on proliferation, apoptosis and migration of A2780 cells. International Journal of Frontiers in Medicine (2022), Vol. 4, Issue 2: 48-54. https://doi.org/10.25236/IJFM.2022.040209.

[1] Li YN, Du XB, Lu FH, et al. (2020) The effect of miR-19b on the invasion and migration in ovarian cancer cells. Journal of Modern Oncology, 28: 9-12.

[2] Siegel RL, Miller KD, Jemal A. (2017) Cancer statistics, 2017 [J]. CA Cancer J Clin, 67(1): 7-30.

[3] Banerjee S, Kaye SB. (2013) New strategies in the treatment of ovarian cancer: current clinical perspectives and future potential [J]. Clin Cancer Res, 19(5): 961-968.

[4] Mezzanzanica D. (2015) Ovarian cancer: A molecularly insidious disease [J]. Chin J Cancer, 34(1): 1-3.

[5] SONG Z, ZHOU Y, BAI X, et al. (2021) A practical nomogram to predict early death in advanced epithelial ovarian ancer [J]. Front Oncol, 11, 655826.

[6] Gurung A, Hung T, Morin J, et al. (2013) Molecular abnormalities in ovarian carcinoma: clinical, morphological and therapeutic correlates [J]. Histopathology, 62(1): 59-70.

[7] Ch. P (2015) VolⅠ, Chinese Pharmacopoeia 2015 Edition · 1 [S]. 2015: 223-225.

[8] Kumar M, Prasad SK, Hemalatha S. (2014) A current update on the phytopharmacological aspects of Houttuynia cordata Thunb [J]. Pharmacogn Rev, 8(15): 22-35.

[9] STEWART C, RALYEA C, LOCKWOOD S. (2019) Ovarian cancer: an integrated review [J]. Semin Oncol Nurs, 35 (2):151-156.

[10] Xu GJ, Li ZJ, Wang Q, et al. (2016) Isolation and identification of anti-inflammatory constituents from Houttuynia cordate [J]. J China Pharm Univ, 47(3):294-298.

[11] Rani N, Bharti S, Krishnamurthy B, et al. (2016) Pharmacological Properties and Therapeutic Potential of Naringenin: A Citrus Flavonoid of Pharmceutical Promise [J]. Curr Pharm Des, 22(28): 41-59.

[12] Jin AY, Song XJ, Huang CC, et al. (2016) Mechanism on the prohibitory effect of Flavonoids on the Resistant Bacteria [J]. Anim Husb Vet Med, 48(12): 117-120.

[13] Li XX, Lian CX, Li XJ, et al. (2016) In vitro antiviral mechanism of euheniin and quercetin against varicella-zoster virus [J]. Chin J Microbiol Immunol, 36(10): 38-42.

[14] Chen YF, Yang JS, Chang WS, et al. (2013) Houttuynia cordata Thunb extract modulates G0/G1 arrest and Fas/CD95-mediated death receptor apoptotic cell death in human lung cancer A549 cells. Journal of Biomedical Science 20: 18.

[15] Yanarojana M, Nararatwanchai T, Thairat S, et al. (2017) Antiproliferative Activity and Induction of Apoptosis in Human Melanoma Cells by Houttuynia cordata Thunb Extract. Anticancer Research 37: 6619-6628.

[16] Zhou NN, Tang J, Chen WD, et al. (2012) Houttuyninum, an active constituent of Chinese herbal medicine, inhibits phosphorylation of HER2/neu receptor tyrosine kinase and the tumor growth of HER2/neu-overexpressing cancer cells. Life Sciences 90: 770-775.

[17] Kim IS, Kim JH, Kim JS, et al. (2007) The inhibitory effect of Houttuynia cordata extract on stem cell factor-induced HMC-1 cell migration. Journal of Ethnopharmacology 112: 90-95.

[18] Duo J, Ying GG, Wang GW, et al. (2012) Quercetin inhibits human breast cancer cell proliferation and induces apoptosis via Bcl-2 and Bax regulation. Molecular Medicine Reports 5: 1453-1456.

[19] Sakthivel KM and Guruvayoorappan C. (2018) Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and inhibition of transcription factor activation. Regulatory Toxicology and Pharmacology 95: 400-411.

[20] Song P, Hai Y, Wang X, et al. (2018) Realgar transforming solution suppresses angiogenesis and tumor growth by inhibiting VEGF receptor 2 signaling in vein endothelial cells. Archives of Pharmacal Research 41: 467-480.

[21] Kim JM, Hwang IH, Jang IS, et al. (2017) Houttuynia cordata Thunb Promotes Activation of HIF-1A-FOXO3 and MEF2A Pathways to Induce Apoptosis in Human HepG2 Hepatocellular Carcinoma Cells. Integrative Cancer Therapies 16: 360-372.