Weizhe Liu1, Juanjuan Zhang2,3, Aiying Li1,2
1Department of Biochemistry and Biology, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, China
2Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease, Shijiazhuang, China
3Key Laboratory for Health Care with Chinese Medicine of Hebei Province, Shijiazhuang, China
Myocardial infarction is a heart disease with high mortality rate. The research on the cardiomyocyte apoptosis is helpful to the prevention and treatment of myocardial infarction. As a cell surface receptor, the function of ITGB1 gene in cardiomyocytes is not clear. This study aimed to analyze the function and molecular mechanism of cardiomyocyte derived ITGB1 in myocardial apoptosis. To analyze cardiomyocyte apoptosis, TUNEL staining was performed with ITGB1 overexpression and knockdown in H9C2 cells. Apoptosis-related markers were also detected using Western blot. In addition, apoptosis-related signaling pathways were detected to elucidate the molecular mechanism regulated by ITGB1. Our results indicated that ITGB1 promoted cardiomyocyte apoptosis by inhibiting the Notch1 and Smad2/3 signal pathways. These data highlight the potential usefulness of ITGB1 as a potential target for preventing myocardial infarction.
ITGB1, H9C2 cells, Cardiomyocyte apoptosis, Myocardial infarction
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