Jing Zhang, Bin Li, Jialan Chen, Fei Wang, Yu Zhang, Lu Gan
Gynecology Department, Shaanxi Provincial People's Hospital/Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China
Background: This study is aim to profile the differentially expressed microRNAs (DEMs) of premenopausal endometrial cancer (EC), identify their target genes and understand their roles in carcinogenesis. Methods: Next-generation sequencing (NGS) was performed on 3 premenopausal EC and 3 premenopausal normal endometrial tissues. Selection of candidate miRNAs and subsequent validation were performed by qRT-PCR on 20 premenopausal EC, 30 premenopausal normal endometrial and 40 postmenopausal EC samples. The relationship between DEMs and clinical characteristics was analyzed. Results: 136 upregulated and 131 downregulated DEMs were identified. The expression of miR-1251-5p was highly upregulated in premenopausal EC samples compared with premenopausal normal endometrial samples and significantly downregulated compared with postmenopausal EC samples. The expression of miR-6892-5p was highly upregulated in premenopausal EC samples compared with premenopausal normal endometrial samples and postmenopausal EC samples. In the premenopausal EC group, miR-1251-5p expression was closely correlated with menarche age, number of pregnancies, tumor grading, myometrial infiltration and lymph node metastasis; miR-6892-5p expression was closely correlated with BMI, hypertension, tumor grading, and metastasis. Conclusions: miR-1251-5p and miR-6892-5p may play important roles in tumorigenesis progression of premenopausal EC.
Premenopausal endometrial cancer; differentially expressed microRNAs; next-generation sequencing; miR-1251-5p; miR-6892-5p
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