International Journal of Frontiers in Medicine, 2022, 4(11); doi: 10.25236/IJFM.2022.041104.
Xuejiao Lian, Yang Yang, Jialing Tang, Xiuwen Li, Xiaoying Zhou
The School of Pharmacy, The School of Medicine, Changzhou University, Changzhou, Jiangsu, 213164, China
Asthma is a common respiratory disease, with chronic inflammatory associated with airway hyperresponsiveness (AHR). Airway smooth muscle (ASM) is the critical tissue modulating bronchial tension. Abnormal activation and proliferation of airway smooth muscle cells (ASMCs) are key process during airway problem development. Histamine, a mast-cell mediator, involves in allergy and air-way inflammation, and stimulates cells via its receptors. Dipotassium Glycyrrhizinate (DG) is a derivative of Glycyrrhizic acid, with anti-inflammation effects, but the effect of DG on histamine-induced ASMC has not discussed yet. Primary rats ASMCs were extracted to establish a histamine/cell-models to investigate the effects of DG on cells, and to explore the potential pharmaceutical mechanism of DG in the treatment of AHR. The data showed that DG inhibited histamine-induced cell proliferation, migration by suppressing P38/ERK and JAK/STAT signal pathway. The data also showed that DG up-regulated Bax expression and down-regulated Bcl-2 expression, leading to the apoptosis of activated ASMCs. Molecular docking analysis illustrated the high binding affinity of DG with histamine receptor, which is verified the antagonist function of DG on histamine by competing over histamine receptor. The present study suggests the anti-histamine function of DG and the underlying mechanism that DG can relieve airway hyper-responsiveness by inhibiting histamine-induced over-activation/proliferation and regulating airway smooth muscle cell intracellular signaling pathways.
airway smooth muscle cells, histamine, dipotassium glycyrrhizinate, airway hyperresponsiveness
Xuejiao Lian, Yang Yang, Jialing Tang, Xiuwen Li, Xiaoying Zhou. Anti-histamine Effects of Dipotassium Glycyrrhizinate on Airway Smooth Muscle Cell, Implicating Its Therapeutic Mechanism for Airway Hyperresponsiveness. International Journal of Frontiers in Medicine (2022), Vol. 4, Issue 11: 22-30. https://doi.org/10.25236/IJFM.2022.041104.
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