Chen Junlin1, Li Rui2
1School of Science& Technology, Hong Kong Metropolitan University, Hong Kong, 999077, China
2School of Graduate Studies, Hong Kong Lingnan University, Hong Kong, 999077, China
A solution containing the ligand is incubated with an immobilized protein of interest on a solid support. Unbound ligands are washed away, and bound ligands are detected. Protein affinity analysis can be used to characterize protein-protein interactions and to screen for inhibitors. Despite being low-throughput, protein microarray/S.E.L.D.I. mass spectrometry (Ciphergen, Fremont, CA, U.S.A.) has been used for differential analysis and protein marker discovery in infectious diseases and cancer. Protein sequencing methods can accurately differentiate ovarian cancer from non-cancers in serum by using proteomic approaches for high-risk and general populations. It can be used to screen for cancer and autoimmune diseases like systemic lupus erythematosus. Thus, high-throughput sequencing technology is widely used in various fields of laboratory medicine, and if some methods and techniques are combined, test results can be accurate and specific.
High-throughput protein arrays; Molecular biology; Proteomics; Immunological assays
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