Cao Xinyang1,2, Zhan Hongju1,2, Zhang Yu1,2, Fang Quantang1,2, Hong Bin1,2, Sun Zijian1,2
1School of Chemical Engineering and Pharmacy, Jingchu Institute of Technology, 448000, Jingmen, China
2Hubei Key Laboratory of Drug Synthesis and Optimization, 448000, Jingmen, China
With the help of DrugBank, TTD and SuperPred, the action targets of lobotropin were screened, and the action targets of gastric cancer were identified by DisGeNET, MalaCards and DigSee, and the two were taken to intersect, and there were 13 identical action targets, namely: HSP90AA1, NFKB1, MMP1, MMP2, OGG1, ABCC1 , CXCR4, MTOR, ESR1, TLR4, AR, NFE2L2 and ITGB1, and the above genes were differentially expressed in gastric cancer by analysis of gastric cancer genes in the TCGA database. Drug-target-disease-pathway mapping, target interaction (PPI) network construction and KEGG and GO analysis of common targets screened five significant genes as NFKB1, TLR4, AR, MTOR and ITGB1, respectively. molecular docking was performed to show that lobeline had binding effect on all the above targets. Gene profiles of GSE14210, GSE15459, GSE22377, GSE29272, GSE51105 and GSE62254 were obtained from the GEO database plotting K-M survival curves indicated that NFKB1, TLR4, AR, MTOR and ITGB1 all had survival differences. Gastric cancer genes downloaded from the TCGA database were immunocorrelated by immunoassay for co-expression targets. The TIMER database was used to show the correlation of NFKB1, TLR4, AR, MTOR and ITGB1 on the direction of immune infiltration of gastric cancer cells. In conclusion lobotropin regulates NFKB1, TLR4, AR, MTOR and ITGB1 in gastric cancer tissues to achieve regulation of tumor progression.
Stomach Cancer, Solanine, Immune infiltration of tumor cells, 1Molecular Docking
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