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Academic Journal of Medicine & Health Sciences, 2023, 4(1); doi: 10.25236/AJMHS.2023.040110.

Exploring the Mechanism of Action and Survival and Immune Analysis of Lobotropin on Gastric Cancer through Network Pharmacology


Cao Xinyang1,2, Zhan Hongju1,2, Zhang Yu1,2, Fang Quantang1,2,  Hong Bin1,2, Sun Zijian1,2

Corresponding Author:
​Cao Xinyang

1School of Chemical Engineering and Pharmacy, Jingchu Institute of Technology, 448000, Jingmen, China

2Hubei Key Laboratory of Drug Synthesis and Optimization, 448000, Jingmen, China


With the help of DrugBank, TTD and SuperPred, the action targets of lobotropin were screened, and the action targets of gastric cancer were identified by DisGeNET, MalaCards and DigSee, and the two were taken to intersect, and there were 13 identical action targets, namely: HSP90AA1, NFKB1, MMP1, MMP2, OGG1, ABCC1 , CXCR4, MTOR, ESR1, TLR4, AR, NFE2L2 and ITGB1, and the above genes were differentially expressed in gastric cancer by analysis of gastric cancer genes in the TCGA database. Drug-target-disease-pathway mapping, target interaction (PPI) network construction and KEGG and GO analysis of common targets screened five significant genes as NFKB1, TLR4, AR, MTOR and ITGB1, respectively. molecular docking was performed to show that lobeline had binding effect on all the above targets. Gene profiles of GSE14210, GSE15459, GSE22377, GSE29272, GSE51105 and GSE62254 were obtained from the GEO database plotting K-M survival curves indicated that NFKB1, TLR4, AR, MTOR and ITGB1 all had survival differences. Gastric cancer genes downloaded from the TCGA database were immunocorrelated by immunoassay for co-expression targets. The TIMER database was used to show the correlation of NFKB1, TLR4, AR, MTOR and ITGB1 on the direction of immune infiltration of gastric cancer cells. In conclusion lobotropin regulates NFKB1, TLR4, AR, MTOR and ITGB1 in gastric cancer tissues to achieve regulation of tumor progression.


Stomach Cancer, Solanine, Immune infiltration of tumor cells, 1Molecular Docking

Cite This Paper

Cao Xinyang, Zhan Hongju, Zhang Yu, Fang Quantang,  Hong Bin, Sun Zijian. Exploring the Mechanism of Action and Survival and Immune Analysis of Lobotropin on Gastric Cancer through Network Pharmacology. Academic Journal of Medicine & Health Sciences (2023) Vol. 4, Issue 1: 56-65. https://doi.org/10.25236/AJMHS.2023.040110.


[1] Gullo I, Grillo F, Mastracci L, Vanoli A, Carneiro F, Saragoni L, Limarzi F, Ferro J, Parente P, Fassan M. Precancerous lesions of the stomach, gastric cancer and hereditary gastric cancer syndromes. Pathologica. 2020 Sep;112(3):166-185. 

[2] Smyth EC, Nilsson M, Grabsch HI, van Grieken NC, Lordick F. Gastric cancer. Lancet. 2020 Aug 29;396(10251):635-648.

[3] Thrift AP, El-Serag HB. Burden of Gastric Cancer. Clin Gastroenterol Hepatol. 2020 Mar;18(3):534-542.

[4] Amashina T, Ishihara R, Nagai K, Matsuura N, Matsui F, Ito T, Fujii M, Yamamoto S, Hanaoka N, Takeuchi Y, Higashino K, Uedo N, Iishi H. Long-term outcome and metastatic risk after endoscopic resection of superficial esophageal squamous cell carcinoma. Am J Gastroenterol. 2013 Apr;108(4):544-51.

[5] Leng JC, Gany F. Traditional Chinese medicine use among Chinese immigrant cancer patients. J Cancer Educ. 2014 Mar;29(1):56-61.

[6] Gao Y, Yu AL, Li GT, Hai P, Li Y, Liu JK, Wang F. Hexacyclic monoterpenoid indole alkaloids from Rauvolfia verticillata. Fitoterapia. 2015 Dec;107:44-48.

[7] Kuo CI, Chao CH and Lu MK: Effects of auxins on the production of steroidal alkaloids in rapidly proliferating tissue and cell cultures of Solanum lyratum. Phytochem Anal. 23:400–404. 2012.

[8] Friedman M, Lee KR, Kim HJ, Lee IS and Kozukue N: Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma and stomach cancer cells. J Agric Food Chem. 53:6162–6169. 2005.

[9] Yang SA, Paek SH, Kozukue N, Lee KR and Kim JA: Alpha-chaconine, a potato glycoalkaloid, induces apoptosis of HT-29 human colon cancer cells through caspase-3 activation and inhibition of ERK 1/2 phosphorylation. Food Chem Toxicol. 44:839–846. 2006.

[10] Lee KR, Kozukue N, Han JS, Park JH, Chang EY, Baek EJ, Chang JS and Friedman M: Glycoalkaloids and metabolites inhibit the growth of human colon (HT29) and liver (HepG2) cancer cells. J Agric Food Chem. 52:2832–2839. 2004.

[11] Lu MK, Shih YW, Chang Chien TT, Fang LH, Huang HC and Chen PS: α-Solanine inhibits human melanoma cell migration and invasion by reducing matrix metalloproteinase-2/9 activities. Biol Pharm Bull. 33:1685–1691. 2010.

[12] Lv C, Kong H, Dong G, Liu L, Tong K, Sun H, Chen B, Zhang C, Zhou M. Antitumor efficacy of α-solanine against pancreatic cancer in vitro and in vivo. PLoS One. 2014 Feb 5;9(2):e87868.

[13] Sun H, Lv C, Yang L, Wang Y, Zhang Q, Yu S, Kong H, Wang M, Xie J, Zhang C and Zhou M: Solanine induces mitochondria-mediated apoptosis in human pancreatic cancer cells. Biomed Res Int. 2014:8059262014.

[14] Ji YB, Gao SY, Ji CF and Zou X: Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein. J Ethnopharmacol. 115:194–202. 2008.

[15] Lu MK, Chen PH, Shih YW, Chang YT, Huang ET, Liu CR and Chen PS: Alpha-Chaconine inhibits angiogenesis in vitro by reducing matrix metalloproteinase-2. Biol Pharm Bull. 33:622–630. 2010.

[16] Awale M, Reymond JL. Web-based 3D-visualization of the DrugBank chemical space. J Cheminform. 2016 May 4;8:25.

[17] Zhang J, Liu X, Zhou W, Cheng G, Wu J, Guo S, Jia S, Liu Y, Li B, Zhang X, Wang M. A bioinformatics investigation into molecular mechanism of Yinzhihuang granules for treating hepatitis B by network pharmacology and molecular docking verification. Sci Rep. 2020 Jul 10;10(1):11448.

[18] Nickel J, Gohlke BO, Erehman J, Banerjee P, Rong WW, Goede A, Dunkel M, Preissner R. SuperPred: update on drug classification and target prediction. Nucleic Acids Res. 2014 Jul;42(Web Server issue):W26-31.

[19] UniProt Consortium. Reorganizing the protein space at the Universal Protein Resource (UniProt). Nucleic Acids Res. 2012 Jan;40(Database issue):D71-5.

[20] Bauer-Mehren A, Rautschka M, Sanz F, Furlong LI. DisGeNET: a Cytoscape plugin to visualize, integrate, search and analyze gene-disease networks. Bioinformatics. 2010 Nov 15;26(22):2924-6.

[21] Rappaport N, Fishilevich S, Nudel R, Twik M, Belinky F, Plaschkes I, Stein TI, Cohen D, Oz-Levi D, Safran M, Lancet D. Rational confederation of genes and diseases: NGS interpretation via GeneCards, MalaCards and VarElect. Biomed Eng Online. 2017 Aug 18; 16(Suppl 1):72.

[22] Kim J, So S, Lee HJ, Park JC, Kim JJ, Lee H. DigSee: Disease gene search engine with evidence sentences (version cancer). Nucleic Acids Res. 2013 Jul; 41(Web Server issue):W510-7.

[23] Bardou P, Mariette J, Escudié F, Djemiel C, Klopp C. jvenn: an interactive Venn diagram viewer. BMC Bioinformatics. 2014 Aug 29; 15(1):293. 

[24] Chandran UR, Medvedeva OP, Barmada MM, Blood PD, Chakka A, Luthra S, Ferreira A, Wong KF, Lee AV, Zhang Z, Budden R, Scott JR, Berndt A, Berg JM, Jacobson RS. TCGA Expedition: A Data Acquisition and Management System for TCGA Data. PLoS One. 2016 Oct 27; 11(10):e0165395.

[25] Piñero J, Saüch J, Sanz F, Furlong LI. The DisGeNET cytoscape app: Exploring and visualizing disease genomics data. Comput Struct Biotechnol J. 2021 May 11; 19: 2960-2967.

[26] Sharobeem S, Boulmier D, Leurent G, Bedossa M, Leclercq C, Mabo P, Martins RP, Tomasi J, Verhoye JP, Donal E, Sost G, Le Guellec M, Le Breton H, Auffret V. Prognostic impact of permanent pacemaker implantation after transcatheter aortic valve replacement. Heart Rhythm. 2022 July; 19(7):1124-1132. 

[27] Yuan F, Lu L, Zhang Y, Wang S, Cai YD. Data mining of the cancer-related lncRNAs GO terms and KEGG pathways by using mRMR method. Math Biosci. 2018 Oct; 304:1-8.

[28] Luo Y, Chen P, Yang L, Duan X. Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Gastrodia elata Blume in the treatment of ischemic stroke. Exp Ther Med. 2022 Nov 3;24(6):742.

[29] Zheng Y, Tu J, Wang X, Yu Y, Li J, Jin Y, Wu J. The Therapeutic Effect of Melatonin on GC by Inducing Cell Apoptosis and Autophagy Induced by Endoplasmic Reticulum Stress. Onco Targets Ther. 2019 Nov 26;12:10187-10198.

[30] Yang J, Gu W, Li Y. Biological enrichment prediction of polychlorinated biphenyls and novel molecular design based on 3D-QSAR/HQSAR associated with molecule docking. Biosci Rep. 2019 May 17;39(5):BSR20180409.

[31] Yuan B, Liu G, Dai Z, Wang L, Lin B, Zhang J. CYP1B1: A Novel Molecular Biomarker Predicts Molecular Subtype, Tumor Microenvironment, and Immune Response in 33 Cancers. Cancers (Basel). 2022 Nov 17; 14(22):5641.