International Journal of Frontiers in Medicine, 2023, 5(3); doi: 10.25236/IJFM.2023.050304.
Tao Jiang1,2, Weixing Lei1,2, Nan Ling1,2, Shuting Yang1,2, Faen An1,2, Guiping Pang1,2, Yuetao Huang1,2, Kunhua Wei3, Meizhou Shen1,2, Qiming Yu1,2
1School of Public Health, Guilin Medical University, Guilin, China
2Guangxi Key Laboratory of Environmental Exposureomics and Entire Lifecycle Health, Guilin Medical University, Guilin, China
3Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Resources, Guangxi Medicinal Botanical Garden, Nanning, China
The acute liver injury model of mice induced by carbon tetrachloride (CCl4) was used to explore the protective effect of aqueous extract of Spatholobi Caulis on acute liver injury. Methods: The contents of total phenols and total flavonoids in the aqueous and ethanol extracts of Caulis spatholobi were determined, and the antioxidant activity was evaluated by DPPH, ABTS free radical scavenging rate and FRAP total reducing capacity. 30 C57BL/6J male mice were randomly divided into model group, bifendate group, the low-dose, medium-dose and high-dose groups of aqueous extract of Spatholobi Caulis (n = 6). The animal model of acute liver injury was established by intraperitoneal injection of CCl4 after 7 days with corresponding drugs. The pathological changes of liver tissue were observed by H&E staining. Serum levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor α (TNF-α) were determined by ELISA. The indexes of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and antioxidant effects of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were determined. Results: Liver histomorphology observation showed that aqueous extract of Spatholobi Caulis could significantly improve liver cell injury and inflammatory cell infiltration, and significantly increased the activities of CAT, SOD and GSH-Px antioxidant enzymes in liver, decreased the contents of MDA and ALT and AST in serum, significantly down-regulated the levels of IL-1β, IL-6 and TNF-α in serum, and up-regulated the levels of IL-10 and other inflammatory factors. Conclusion: The aqueous extract of Spatholobi Caulis can improve acute liver injury induced by CCl4 in mice through improving antioxidant and anti-inflammatory effects.
Spatholobi Caulis, Aqueous extract, Acute liver injury, CCl4
Tao Jiang, Weixing Lei, Nan Ling, Shuting Yang, Faen An, Guiping Pang, Yuetao Huang, Kunhua Wei, Meizhou Shen, Qiming Yu. Antioxidant Activity of Aqueous Extract of Spatholobi Caulis and Its Ameliorative Effect on CCl4 Induced Acute Liver Injury in Mice. International Journal of Frontiers in Medicine (2023), Vol. 5, Issue 3: 20-27. https://doi.org/10.25236/IJFM.2023.050304.
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