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International Journal of Frontiers in Medicine, 2023, 5(3); doi: 10.25236/IJFM.2023.050306.

Application of Metabolomics in Oral Cancer


Jie Bai, Zhitao Yao

Corresponding Author:
Zhitao Yao

Department of Oral and Maxillofacial Trauma and Orthognathic Surgery, the First Affiliated Hospital (Stomatological Hospital) of Xinjiang Medical University, Urumqi, China


As an extension and terminal of genomics and proteomics, metabolomics has recently become a research focus of disease prevention, pathogenesis and disease diagnosis. With the development of metabolomics, its research in oral cancer and potential malignant lesions of oral cavity can provide important reference materials for laboratory and clinical practice. This paper reviews the theoretical basis of metabolomics, detection methods and analysis methods in oral cancer and potential malignant lesions of oral cavity.


metabolomics; oral cancer

Cite This Paper

Jie Bai, Zhitao Yao. Application of Metabolomics in Oral Cancer. International Journal of Frontiers in Medicine (2023), Vol. 5, Issue 3: 34-38. https://doi.org/10.25236/IJFM.2023.050306.


[1] Zhou Zengtong, Liu Wei. Prediction of cancer risk in oral precancerous lesions [J]. Journal of Practical Oncology, 2015, 30 (1): 4-6.

[2] Zhou J, Xu B, Huang J, et al. 1H NMR - based metabonomic and pattern recognition analysis for detection of oral squamous cell carcinoma [J]. Clin Chim Acta, 2009, 401(1): 8-13.

[3] Liu S A,Tsai W C,Wong Y K,et al. Nutritional factors and survival of patients with oral cancer[J]. Head Neck, 2006, 28(11): 998 - 1007.

[4] Cheng Xingqun, Deng Meng, Xu Xin, et al. Saliva and salivomics and early diagnosis of disease [J]. International Journal of Oral Medicine, 2014, 41 (02): 213-219.

[5] Li Qian, Zhang Ping, Chen Jiao, et al. Application of salivomics in the diagnosis of oral cancer [J]. International Journal of Oral Medicine, 2018, 45 (06): 710-715.

[6] Xue Jiayi, Zhu Xiaoyan, Wei Changlei, et al. Saliva metabolomic analysis of 25 patients with oral squamous cell carcinoma [J]. Shanghai Stomatology, 2021, 30 (02): 201-205.

[7] JESSRI M, DALLEY A J, FARAH C S.MutSα and MutLα immuno expression analysis indiagnostic grading of oral epithelial dysplasia and squamous cell carcinoma[J]. Oral Surg OralMed Oral Pathol Oral Radiol, 2015, 119(1):74-82.

[8] KALMAR J R.Advances in the detection and diagnosis of oral precancerous and cancerous lesions[J].Oral & Maxillofacial Surgery Clinics of North America, 2006,18(18): 465-482.

[9] DIXON R A, GANG D R, CHARLTON A J, et al.Applications of metabolomics in agriculture[J]. Journal of Agricultural & Food Chemistry, 2006, 54(24): 8984-94.

[10] HERMAN C, CETINGUL M P.Quantitative visualization and detection of skin cancer using dynamic thermal imaging [J]. Journal of Visualized Experiments Jove, 2010, 51(51):e2679-e2679.

[11] NAVAZESH M.Methods for collecting saliva [J].Annals of the New York Academy of Sciences,2010, 694(1): 72-77.

[12] WIKLUND S, JOHANSSON E, SJOESTROEM L, et al.Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models [J]. Analytical chemistry, 2008, 80(1): 115-22.

[13] SACCENTI E, HOEFSLOOT H C J, SMILDE A K, et al.Reflections on univariate and multivariate analysis of metabolomics data[J].Metabolomics, 2014, 10(3):361-374.

[14] KANEHISA M, GOTO S.KEGG: kyoto encyclopedia of genes and genomes [J]. Nucleic Acids Res, 2000, 28(1): 27-30.

[15] KANEHISA M, SATO Y, KAWASHIMA M, et al.KEGG as a reference resource for gene and protein annotation [J].Nucleic acids research, 2015, gkv1070.

[16] XIA J, SINELNIKOV I V, HAN B, et al.MetaboAnalyst 3.0—making metabolomics more meaningful [J]. Nucleic acids research, 2015, 43(W1): W251-W7.

[17] KOLDE R. Pheatmap: pretty heatmaps [J]. R package version, 2012, 61.

[18] BINNE N J, WONG D T.Salivary biomarker development using genomic, proteomic and metabolomic approaches [J]. Genome Medicine, 2012, 4(10):1-12.

[19] TIZIANI S, LOPES V, GUNTHER U L.Early stage diagnosis of oral cancer using 1HNMR-based metabolomics[J]. Neoplasia, 2009, 11(3):269, IN7-276,IN10.

[20] Li Xiaojuan, Peng Tao, Chen Dongdong, et al. Progress in the application of mass spectrometry in the analysis of pesticide residues in food samples [J]. Journal of Pesticide, 2011, 13 (6): 555-567.

[21] Cheng Y, Wright J: Advances in diagnostic adjuncts for oral squamous cell carcinoma. The Open Pathology Journal 2011, 5:3-7. Master's dissertation of Qingdao University 23

[22] RM: Saliva as a tool for oral cancer diagnosis and prognosis.Oral Oncol 2009, 45(12):1006-10.

[23] Rai, VertikaMukherjee, RashmiGhosh, Ananta K. Routray, AurobindaChakraborty, Chandan. "Omits" in oral cancer: New approaches for biomarker discovery [J]. Archives of Oral Biology, 2018, 87.

[24] Feizi, NedaHashemi-Nasab, Fatemeh SadatGolpelichi, FatemehSaburouh, NazaninParastar, Hadi. Recent trends in application of chemometric methods for GC-MS and GCxGC-MS-based metabolomic studies [J]. TrAC: Trends in Analytical Chemistry, 2021, 138(1).

[25] Xue Jiayi. Saliva metabolomics in patients with oral squamous cell carcinoma [D]. Qingdao University, 2018.

[26] Yu Xiaodi, Sun Hongying. Metabolomics in the study of oral cancer and oral precancerous lesion markers [J]. Oral Medicine, 2017, 37 (04): 369-372.

[27] Fonville JM, Maher AD, Coen M, et al. Evaluation of full-resolution J-resolved 1H NMR projections of biofluids for metabonomics information retrieval and biomarker identification[J]. Anal Chem, 2010, 82 (5): 1811-1821.

[28] Somashekar B S, Kamarajan P, Danciu T, et al. Magic angle spinning NMR based metabolic profiling of head and neck squamous cell carcinoma tissues[J]. Journal of Proteome Research, 2011,10 (11): 523-5241.

[29] Winiarska K, Szymanski K, Gorniak P, et al. Hypoglycaemic, antioxidative and nephroprotective effects of taurine in alloxan diabetic rabbits [J]. Biochimie, 2009, 91(2): 261-270.

[30] Yan S K, Wei B J, Lin Z Y, et al. A metabonomic approach to the diagnosis of oral squamous cell carcinoma, oral lichen planus and oral leukoplakia [J]. Oral Oncology, 2008, 44(5): 477 - 483.

[31] Wei J, Xie G, Zhou Z, et al. Salivary metabolite signatures of oral cancer and leukoplakia [J]. Int J Cancer, 2011, 129(9): 2207 - 2217.

[32] Gupta A, Gupta S, Mahdi A A. 1H NMR - derived serum metabolomics of leukoplakia and squamous[J]. Clin Chim Acta, 2015, 441(2): 47 - 55.

[33] Tiziani S, Lopes V, Günther U L. Early-Stage Diagnosis of oral cancer using 1H NMR - Based metabolomics[J]. Neoplasia, 2009, 11 (3): 269 - 276.

[34] Ganti S, Taylor S L, Aboud O A, et al. Kidney Tumor Biomarkers Revealed by Simultaneous Multiple Matrix Metabolomics Analysis [J]. Cancer Research, 2012, 72 (14): 3471-3479.

[35] Anlauf E, Derouiche A. Glutamine synthetase as an astrocytic marker: Its cell type and vesicle localization [J]. Front Endocrinol (Lausanne), 2013, 16(4): 144.

[36] Musharraf S G, Shahid N, Naqvi S M, et al. Metabolite profiling of preneoplastic and neoplastic lesions of oral cavity tissue samples revealed a biomarker pattern[J]. Scientific Reports, 2016,6: 38985.

[37] Matthew G, Vander H, Lewis C, et al. Understanding the warburg effect: the metabolic requirements of cell proliferation [J]. Science, 2009, 324(5930): 1029 - 1033.