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International Journal of Frontiers in Medicine, 2023, 5(3); doi: 10.25236/IJFM.2023.050307.

Progress in the study of four dental age inference methods in the age inference of children and adolescents


Duan Sichen, Geng Jiwen, Wang Yu, Liu Jia

Corresponding Author:
Liu Jia

Department of Pediatric Dentistry, First Affiliated Hostipal (Affiliated Stomatological Hostipal) of Xinjiang Medical University, Urumqi, 830054, China


The age of the human individual is an important element of the individual's biological information. Over time, many irreversible ageing changes occur in several organs and tissues of the human body, and in forensic medicine, age interpretation has an extremely important link to the identification of identity in criminal cases, the division of sentencing in juvenile and child crime[1], and the determination of the age of lost persons in missing persons. In previous studies, bone age such as wrist bone, vertebral bone and sternum are often used for age prediction. Bone age can be used as a preliminary evaluation of age, but due to the influence of congenital genetics, racial differences and acquired environment, it may result in poorly developed bone tissue, bringing interference to the process of interpretation and eventually leading to distorted data [2]. The tooth is the hardest human tissue and its tissue structure also contributes to its extremely high stability. Compared to skeletal maturation, teeth are less likely to be altered during growth and development due to changes in the external environment [3-4], which allows it to be used more often in events that require individual identity interpretation. One of the most common methods of estimating dental age is radiological examination to assess the degree of calcification [5], and imaging of the oral cavity is also very common in modern clinical medicine. This article reviews and summarises the research progress and analyses the advantages and disadvantages of four different methods of age estimation applicable to children and adolescents, and objectively evaluates the four different methods of dental age estimation.


dental age, chronological age, orthopantomograms

Cite This Paper

Duan Sichen, Geng Jiwen, Wang Yu, Liu Jia. Progress in the study of four dental age inference methods in the age inference of children and adolescents. International Journal of Frontiers in Medicine (2023), Vol. 5, Issue 3: 39-45. https://doi.org/10.25236/IJFM.2023.050307.


[1] Huang Wenlin. Investigation and research on the minimum age of criminality in China [J]. Science and Research, 2015(6):114. 

[2] Nicholas J, Douglas K, Waters W, et al.Ultrasound evaluation of bone age in rural ecuadorian children and its association with nutrition.Current developments in nutrition, 2019, 3:103-105.

[3] Cardoso HF.Environmental effects on skeletal versus dental development: using a documented subadult skeletal sample to test a basic assumption in human osteological research[J]. Am J Phys Anthropol, 2007, 132(2): 223-233.

[4] Conceicao EL, Cardoso HF.Environmental effects on skeletal versus dental development Ⅱ: further testing of a basic assumption in human osteological research [J]. Am J Phys Anthropol, 2011, 144(3): 463-470.

[5] Sehrawat JS and Singh M.Willems method of dental age estimation in children:a systematic review and meta-analysis.J Forensic Legal Med2017, 52:122-129. 

[6] Schmeling A, Grundmann C, Fuhrmann A, et al.Criteria for age estimation in living individuals. Int J Legal Med 2008, 122(6):457-460.

[7] Martin DD, Wit JM, Hochberg Z, et al.The use of bone age in clinical practice part 2. Horm Res Paediatr 2011, 76(1):10-16. 

[8] Martin DD, Wit JM, Hochberg Z, et al.The use of bone age in clinical practice part 1. Horm Res Paediatr 2011, 76(1):1-9. 

[9] Spadoni GL, Cianfarani S.Bone age assessment in the workup of children with endocrine disorders. Horm Res Paediatr 2010, 73(1):2-5.

[10] Sopher AB, Jean AM, Zwany SK, et al.Bone age advancement in prepubertal children with obesity and premature adrenarche: possible potentiating factors. Obesity (Silver Spring) 2011, 19(6): 1259-1264.

[11] Engebretsen L, Steffen K, Bahr R, et al.The International Olympic Committee Consensus statement on age determination in high-level young athletes. Br J Sports Med 2010, 44(7):476-484. 

[12] Matina RM, Rogol AD. Sport training and the growth and pubertal maturation of young athletes. Pediatr Endocrinol Rev 2011, 9(1):441-455. 

[13] Georgopoulos NA, Roupas ND, Theodoropoulou A, et al.The influence of intensive physical training on growth and pubertal development in athletes. Ann N Y Acad Sci 2010, 1205:39-44.

[14] Bean, R.B.1914 Eruption of teeth as physiological standard for testing development. Pedagog. Sem. 21:596-614. 

[15] Beik, A.K.1913 Physiological age and school entrance.Pedagog.Sem.20: 283-30. 

[16] Catell, P.1928 Dentition as measure of maturity.Cambridge: Harvard University Press. Harvard Monographs in Education, No.9. 

[17] Celikoglu M, Cantekin K, Ceylan I. Dental age assessment: the applicability of Demirjian method in eastern Turkish children. J Forensic Sci 2011, 56 Suppl 1:S220-222.

[18] DEMIRJIAN A, GOLDSTEIN H, TANNER J M.A new system of dental age assessment[J]. HumBiol, 1973, 45(2):211-227.

[19] Miloglu, O., Celikoglu, M., Dane, A., Cantekin, K., & Yilmaz, A. B. (2011). Is the assessment of dental age by the Nolla method valid for eastern Turkish children?Journal of Forensic Sciences, 56(4), 1025-1028.

[20] Asab SA, Noor SNFM and Khamis MF.The accuracy of Demirjian method in dental age estimation of Malay children.Sing Dent J2011, 32:19-27.

[21] Carneiro JL, Caldas IM, Afonso A, et al. Is Demirjian’s original method really useful for age estimation in a forensic context? Forensic Sci Med Pathol. 2015, 11:216-221.

[22] Erdem PA, Yamac E, Erdem MA, et al. A new method to estimate dental age.Acta Odontol Scand2013, 71:590-598.

[23] Kurita, L. M., Menezes, A. V., Casanova, M. S., & Haiter-Neto, F. (2007). Dental maturity as an indicator of chronological age: Radiographic assessment of dental age in a Brazilian population. Journal of Applied Oral Science: Revista FOB, 15(2), 99-104.

[24] Ifesanya JU and Adeyemi AT.Accuracy of age estimation using Demirjian method among Nigerian children.Afr J Med Sci2012, 41:297-300. 

[25] Tomas, L. F., Monico, L. S., Tomas, I., Varela-Patino, P., & Martin-Biedma, B. (2014). The accuracy of estimating chronological age from Demirjian and Nolla methods in a Portuguese and Spanish sample. BMC Oral Health, 14, 160.

[26] Nur, B., Kusgoz, A., Bayram, M., Celikoglu, M., Nur, M., Kayipmaz, S., & Yildirim, S.(2012). Validity of demirjian and nolla methods for dental age estimation for Northeastern Turkish children aged 5-16 years old. Medicina Oral, Patologia Oral Y Cirugia Bucal, 17(5), e871-877.

[27] Wolf GT, Briseno-Marroquin B and Callaway A.Dental age assessment in 6-to 14-year-old German children: comparison of Cameriere and Demirjian methods, Wolf et al.BMC Oral Health.2016, 16: 120. 

[28] Ye X, Jiang F, Sheng X, et al.Dental age assessment in 7–14-year-old Chinese children: comparison of Demirjian and Willems methods. Forensic Sci Int2014; 244: 36–41. 

[29] Fan Jianlin, Zhou Wenlian. Application of the Demirjian method to determine the dental age of children aged 6-16 years in Beijing[J]. Dentistry, 2005, 25(3):179-181.

[30] Chen JW, Liu ZP, Guo JJ, et al. Demirjian method to determine the dental age of adolescents aged 8-16 years in Chengdu[J]. International Journal of Stomatology, 2009, 36(3):273-273.

[31] Liu Bo, Zhang Shuang, Shao Jiao, et al. Correlation between second molar calcification and bone age in children and adolescents in Harbin[J]. Journal of Harbin Medical University, 2015, 49(1): 55-59.

[32] Willems G, Van Olmen A, Spiessens B, et al.Dental age estimation in Belgian children: Demirjian’s technique revisited.J Forensic Sci.2001, 46:893-895. 

[33] Ye X, Jiang F, Sheng X, et al. Dental age assessment in 7–14-year-old Chinese children: Comparison of Demirjian and Willems methods[J]. Forensic Sci Int, 2014, 244:36-41. 

[34] Maber M, Liversidge HM, Hector MP. Accuracy of age estimation of radiographic methods using developing teeth[J]. Forensic Sci Int, 2006, 159(1):S68-S68. 

[35] Ozveren N and Serindere G.Comparison of the applicability of Demirjian and Willems methods for dental age estimation in children from the Thrace region, Turkey.Forensic Sci Int2018, 285:38-43. 

[36] Apayadin BK and Yasar F.Accuracy of the Demirjian,Willems and Cameriere methods of estimating dental age on Turkish children.Nigeria Journal of Clinical Practice 2018, 21:257-263. 

[37] Mani SA, Naing L, John J, et al.Comparison of two methods of dental age estimation in 7–15-year-old Malays.Int J Paediatr Dent2008, 18:380-388. 

[38] Nik-Hussein NN, Kee KM and Gan P.Validity of Demirjian and Willems methods for dental age estimation for Malaysian children aged 5–15 years old.Forensic Science Int2011, 204: 208–e1. 

[39] Kumaresan R, Cugati N, Chandrasekaran B, et al.Reliability and validity of five radiographic dental age estimation methods in a population of Malaysian children.J Investigative Clin Dent2014, 7(1):102-109.

[40] Hegde S, Patodia A and Dixit U.A comparison of the validity of the Demirjian, Willems, Nolla and Haavikko methods in determination of chronological age of 5–15 year-old Indian children.J Forensic and Legal Med2017, 50:49-57. 

[41] Hegde S, Patodia A, Kanksha S, et al. The applicability of the Demirjian, Willems and Chaillet standards to age estimation of 5–15 year old Indian children.Journal of Forensic Odonto-stomatology. 2019, 37(1): 40-50. 

[42] Jian Wang, Xuebing Bai, Miaochen Wang et al. Applicability and accuracy of Demirjian and Willems methods in a population of Eastern Chinese subadults.Forensic Science International. 2018, 8:121-130. 

[43] Y. Zhai, H.Park, J.Han, H. Wang, F. Ji, J. Tao, Dental age assessment in a northern Chinese population, J Forensic Leg Med. 38 (2016) 43-9. 

[44] Chaillet N, Willems G, Demirjian A.Dental maturity in Belgian children using Demirjian's method and polynomial functions: new standard curves for forensic and clinical use. [J]. Forensic Odontostomatol. 2004 Dec; 22(2):18-27. 

[45] Galić I, Vodanović M, Janković S, Mihanović F, Nakaš E, Prohić S. Dental age estimation on Bosnian–Herzegovinian children aged 6–14 years: Evaluation of Chaillet's international maturity standards. J Forensic Leg Med. 2013, 20(1):40-5.

[46] Cruz-Landeira A, Linares-Argote J, Martínez Rodríguez M, Rodríguez-Calvo MS, Otero XL, Concheiro L. Dental age estimation in Spanish and Venezuelan children. Comparison of Demirjian and Chaillet’s scores. Int J Legal Med. 2010 Mar; 124(2):105-12.

[47] Urzel V, Bruzek J. Dental age assessment in children: a comparison of four methods in a recent French population. J Forensic Sci. 2013 Sep; 58(5):1341-7.

[48] Morris Tidball-Binz.Missing people, DNA analysis and identification of human remains. A guide to best practice in armed conflicts and other situations of armed violence. 2nd ed. 2009. 

[49] Sarkar S, Kailasam S, Kumar PM. Accuracy of estimation of dental age in comparison with chronological age in Indian population – A comparative analysis of two formulas.J Forensic Leg Med 2013, 20(4):230-233. 

[50] Bunyarit S S, Nambiar P, Naidu M K, et al.Dental age estimation of Malay children and adolescents: Chaillet and Demirjian's data improved using artificial multilayer perceptron neural network [J]. Pediatric Dental Journal, 2021, 31(2):176- 185. 

[51] Agrawal NK, Hackman L, Dahal S.Dental age assessment using demirjian's eight teeth method and Willems method in a tertiary hospital.J Nepal Med Assoc JNMA 2018, 56:912e6. 

[52] Bunyarit SS, Jayaraman J, Naidu MK, Yuen Ying RP, Nambiar P, Asif MK. Dental age estimation of Malaysian Chinese children and adolescents: Chaillet and Demirjian's method revisited using artificial multilayer perceptron neural network. Aust J Forensic Sci 2020, 52:681e98. 

[53] Nolla C M.The development of permanent teeth [D].University of Michigan, 1952. 

[54] Kumaresan, R., Cugati, N., Chandrasekaran, B., & Karthikeyan, P. (2016). Reliability and validity of five radiographic dental-age estimation methods in a population of Malaysian children. Journal of Investigative and Clinical Dentistry, 7(1), 102-109.

[55] Mohammed, R. B., Sanghvi, P., Perumalla, K. K., Srinivasaraju, D., Srinivas, J., Kalyan, U. S., & Rasool, S. M. (2015). Accuracy of four dental age estimation methods in southern Indian children. Journal of Clinical and Diagnostic Research: JCDR, 9(1). HC01-08.