Frontiers in Medical Science Research, 2019, 1(4); doi: 10.25236/FMSR.2019.010402.
Jingjing Li 1, Peng Cai 1, Bin Peng 1, Guiming Chen 1,*
1 Department of Oncology I, Jingmen No.2 People's Hospital, Jingmen Hubei 448000, China
*Corresponding Author
Objective: To investigate the application of quantum dot labeled bimolecular probes in the analysis of synergistic expression of HER-2 and Ki-67 in breast cancer. Methods: Quantum dot labeled dual-color fluorescence imaging, image acquisition, spectral separation and quantitative separation of biomarkers. Results: HER-2 showed green fluorescence in breast cancer cell membrane and Ki-67 showed red fluorescence in cancer cell nucleus. The contrast between HER-2 and Ki-67 was obvious, which was helpful for quantitative analysis by spectral stripping. The median 8-DFS was 11.7 months (7.0-26.6 months) and 89.7 months (7.4-96.0 months) in the high and low expression group of Ki-67 (P<0.01). The median 8-DFS in the high HER-2 and high Ki-67 subgroups was shorter than that in the high HER-2 and low Ki-67 subgroups [11.7 months (95% CI: 11.0-26.6) vs 60.1 months (95% CI: 7.4-96.0 months), P < 0.05]; the low HER-2 and high Ki-67 subgroups were significantly shorter than the low HER-2 and low Ki-67 subgroups [16.4 months (95% CI: 6.97-25.67) vs 96.0 months. (95% CI: 16.0-96.0), P < 0.01); high HER-2 high Ki-67 group was significantly shorter than low HER-2 low Ki-67 group [11.7 months (95% CI: 11.0-26.6) vs 96.0 months (95% CI: 16.0-96.0), P < 0.01]; high and low HER-2 group had no significant difference compared with high Ki-67 high HER-2 group [11.7 months (95% CI: 11.0-26.6)] vs 96.0 months (95% CI: 16.0-96.0), P < 0.01]. CI: 11.0-26.6) vs 16.4 months (95% CI: 6.97-25.67), P = 0.586). Conclusion: Quantum dot labeled probe technique is helpful to quantitatively analyze the synergistic expression of HER-2 and Ki-67 in breast cancer.
Quantum dot labeled bimolecular probe; Breast cancer; HER-2; Ki-67; Co-expression
Jingjing Li, Peng Cai, Bin Peng, Guiming Chen. Application of Quantum Dot Labeled Bimolecular Probe in the Analysis of Co-Expression of Her-2 and Ki-67 in Breast Cancer. Frontiers in Medical Science Research (2019) Vol. 1 Issue 4: 7-12. https://doi.org/10.25236/FMSR.2019.010402.
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