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Academic Journal of Materials & Chemistry, 2023, 4(7); doi: 10.25236/AJMC.2023.040709.

Research progress in the resource reuse of distiller's grains


Chuanyao Huang1, Jing Wang1, Jia Zheng2, Jian Su2, Qijun Li2, Yongmei Chen1

Corresponding Author:
Yongmei Chen

1College of Chemical Engineering, Sichuan University of Science & Engineering, Zigong, 643000, China

2Yibin Wuliangye Co., Ltd, Yibin, 644000, China


Distiller's grains are the main by-products produced in the process of brewing and ethanol production. Although they are rich in nutrients, they are highly susceptible to spoilage. Their unscientific utilization not only causes environmental pollution, but also leads to huge waste of resources. Therefore, it is urgent to carry out research on the resource utilization of distiller's grains. In recent years, various studies have been conducted on the resource utilization of distiller's grains both domestically and internationally. According to the utilization methods, it can be mainly divided into four categories: energy production, agricultural products, food, and chemical raw materials, including the production of biogas, animal feed, flour products, and succinic acid, to achieve the resource utilization of distiller's grains. This article reviews the nutritional composition, production and utilization status of distiller's grains, as well as the challenges and future development directions faced by the resource utilization of distiller's grains, in order to provide reference for the treatment and technical research of distiller's grains.


Distillers’ grains; Resource utilization; Nutritional composition; Technical study

Cite This Paper

Chuanyao Huang, Jing Wang, Jia Zheng, Jian Su, Qijun Li, Yongmei Chen. Research progress in the resource reuse of distiller's grains. Academic Journal of Materials & Chemistry (2023) Vol. 4, Issue 7: 49-59. https://doi.org/10.25236/AJMC.2023.040709.


[1] Mingkun Gao, Guangyu Wen, Fang Qian. Research Progress on the Transformation and Utilization of Baijiu Distillers by Microorganisms[J]. Food and Machinery, Vol. 34 (2018) No. 2, p. 191-194.

[2] Qiong Luo, Junjie Cao, Ling Guo, et al. Analysis of comprehensive utilization of brewing by-products from the perspective of circular economy[J]. Journal of Brewing Science and Technology, Tol. 333 (2022) NO. 3, p. 111-115.

[3] Michele De Luca, Donatella Restuccia, Umile Gianfranco Spizzirri, et al. Wine Lees as Source of Antioxidant Molecules: Green Extraction Procedure and Biological Activity[J]. Antioxidants (Basel), Vol. 12 (2023) No. 3, p. 622-640.

[4] María José Jara-Palacios. Wine Lees as a Source of Antioxidant Compounds[J]. Antioxidants (Basel), Vol. 8 (2019) No. 2, p. 45-55.

[5] Qi Lu, Qingyuan Luo, Jiaxuan Li, et al. Evaluation of the Chemical Composition, Bioactive Substance, Gas Production, and Rumen Fermentation Parameters of Four Types of Distiller's Grains[J]. Molecules, Vol. 27 (2022) No. 18, p. 2369-2376.

[6] Qian Li, Chaoxi Pei, Zhisheng Wang, et al. Comparative study on nutrient composition differences of different types of sake lees[J]. Chinese Journal of Animal Nutrition, Vol. 30 (2018) No. 6, p. 2369-2376.

[7] Jie Yang, Zhenzhen Zhang, Xiaoyuan Ding. Multiple responses optimization of antioxidative components extracted from distiller's grains using response surface methodology and identify their chemical compositions[J]. Journal of Food Processing and Preservation, Vol. 45 (2021) No. 11, p. 15885-15598.

[8] Zhijing Ye, Yunxuan Qin, Roland Harrison et al. Characterization of Bioactive Compounds in Lees from New Zealand Wines with Different Vinification Backgrounds[J]. Antioxidants, Vol. 11 (2022) No. 12, p. 2335-2354.

[9] Jing Bi, Yue Feng, Jing Wang, et al. Effects of Solid-State Fermentation on Total Phenol Content and Antioxidant Activity of White Distiller's Grains[J]. Guangdong Chemical Industry, Vol. 49 (2022) No.23, p. 102-105.

[10] Szymon Głowacki, Agnieszka Salamon, Mariusz Sojak, et al. The Use of Brewer's Spent Grain after Beer Production for Energy Purposes[J]. Materials , Vol. 15 (2022) No.10, p. 3703-3721.

[11] Magdalena Zieli nska, Katarzyna Bułkowska, Wioleta Mikucka. Valorization of Distillery Stillage for Bioenergy Production: A Review[J]. Energies, Vol. 14 (2021) No. 21, p 7235-7252.

[12] Manyuchi, M.M.; Frank, R. Techno-economic feasibility of using sorghum brewers spent grain to generale one megawatt of electricity using direct combustion technology. In Proceedings of the 47th Ghana Institution of Engineers (GhIE) Annual Conference, Accra, Ghana, 29–30 March 2016; p. 45–51.

[13] Wilkinson. S, Smart. K.A, James. S, et al. Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach. Bioenerg. Res. 2016, 10, p. 146–157.

[14] Mussatto, S.I. Brewer’s spent grain: A valuable feedstock for industrial applications. J. Sci. Food Agric. Vol. 94 (2014) No. 7, p. 1264–1275.

[15] Cater. M, Fanedl. L, Malovrh. Š, et al. Biogas production from brewery spent grain enhanced by bioaugmentation with hydrolytic anaerobic bacteria. Bioresour. Technol. Vol. 186 (2015) No. 17, 186, p. 261–269.

[16] Reis C E R, Valle G F, Bento H B S, et al. Sugarcane by-products within the biodiesel production chain: Vinasse and molasses as feedstock for oleaginous fungi and conversion to ethyl esters[J]. Fuel, Vol. 277 (2020), p. 118064-118072.

[17] Andong Song, Jianwei Zhang, Yunhan Wu, et al. Experimental study on production of fuel ethanol by fermentation of distiller's lees biomass[J].Transactions of the Chinese Society of Agricultural Engineering, Vol.4 (2003), p. 278-281.

[18] Wilkinson, S.; Smart, K.A.; Cook, D.J.A. Comparison of Dilute Acid- and Alkali-Catalyzed Hydrothermal Pretreatments forBioethanol Production from Brewers’ Spent Grains. J. Am. Soc. Brew. Chem. Vol. 72 (2014), p. 143–153.

[19] Shang-Chun Z , Hai-Quan Y , Wei Z .Recent Progress on the Utilization of Distiller's Grains: a Review[J].The Food Industry, 2016.

[20] Agarwal, M.; Dinker, A. Application of consolidate enzymatic system of Fusarium and Saccharomyces to enhance the productionof ethanol from spent grain[J]. Renew. Sustain. Energy, Vol. 5 (2013) No. 5, p. 053112-053123.

[21] Daiana V. Trapé, Olivia V. López, Marcelo A. Villar. Vinasse: from a residue to a high added value biopolymer[J]. Bioresources and Bioprocessing, Vol. 8 (2021) No. 1, p. 130-142.

[22] Jizhi Wan, Yunwei Leng, Genjiang Wu, et al. Research on the comprehensive use of rice distillers lees to produce vinegar. China Brewing, Vol. 35 (2016) No. 1, p. 170-173.

[23] Lijing Geng, Yan Zhang, Wei Zhou. Study on comprehensive utilization of rice distiller's lees to produce vinegar[J]. Science and Technology of Food Industry, Vol. 34 (2013) No. 9, p. 190-192+289.

[24] Chao Zhang, Ruiqi Xue, Hui Liao,et al. Study on the process of brewing vinegar by mixing wine lees with wine tail and yellow water[J]. China Brewing, Vol. 42 (2023) No. 6, p. 192-197.

[25] Ancut,a Chetrariu, Adriana Dabija. Spent Grain: A Functional Ingredient for Food Applications[J]. Foods, Vol. 12 (2023) No. 7, p. 1533-1551.

[26] Nocente, F.; Natale, C.; Galassi, E.; Taddei, F.; Gazza, L. Using Einkorn and Tritordeum Brewers’ Spent Grain to Increase the Nutritional Potential of Durum Wheat Pasta. Foods Vol. 10 (2021) No. 1, p. 502-519.

[27] Joncer Naibaho, Małgorzata Korzeniowska, Elisa Julianti, et al. Campaign education and communication to the potential consumers of brewers' spent grain (BSG)-added food products as sustainable foods[J]. Heliyon, Vol. 9 (2023) No. 8, p. 19169-19177.

[28] Ktenioudaki, A.; Alvarez-Jubete, L.; Smyth, T.J.; Kilcawley, K.; Rai, D.K.; Gallagher, E. Application of Bioprocessing Techniques(Sourdough Fermentation and Technological Aids) for Brewer’s Spent Grain Breads. Food Res. Int. Vol. 73 (2015), p. 107–116.

[29] Ginindza, A.; Solomon, W.K.; Shelembe, J.S.; Nkambule, T.P. Valorisation of Brewer’s Spent Grain Flour (BSGF) through WheatMaize-BSGF Composite Flour Bread: Optimization Using D-Optimal Mixture Design. Heliyon, Vol. 8 (2022) No. 2, p. 09514-09528.

[30] Kirssel, L.; Prentice, N. Protein and Fiber Enrichment of Cookie Flour with Brewers Spent Grain. Cereal Chem. Vol. 56 (1979), p. 261–266.

[31] Ktenioudaki, A.; Crofton, E.; Scannell, A.G.M.; Hannon, J.A.; Kilcawley, K.N.; Gallagher, E. Sensory Properties and Aromatic Composition of Baked Snacks Containing Brewer’s Spent Grain. J. Cereal Sci. Vol. 57 (2013), p. 384–390.

[32] Stojceska, V.; Ainsworth, P.; Plunkett, A.; Ibanoglu, S. The Recycling of Brewer’s Processing by-Product into Ready-to-Eat Snacks ˇUsing Extrusion Technology. J. Cereal Sci. Vol. 47 (2008), p. 469–479.

[33] Silbir S, Goksungur Y. Natural red pigment production by Monascus purpureus in submerged fermentation systems using afood industry waste: brew spent grain. Foods, Vol 8 (2019) No. 5, p. 161-172.

[34] Naibaho, J, Butula, N, Jonuzi, E. et al. The Roles of Brewers’ Spent Grain Derivatives inCoconut-Based Yogurt-Alternatives: Microstructural Characteristic and the Evaluation of Physico-Chemical Properties duringthe Storage. Curr. Res. Food Sci. Vol. 5 (2022), p. 1195–1204.

[35] Radhakrishna Shetty, Frederik Riddersholm Petersen, Raju Podduturi b, et al. Fermentation of brewer's spent grain liquids to increase shelf life and give an organic acid enhanced ingredient[J]. Lwt, Vol. 182 (2023), p. 114911-114921.

[36] Arranz, J.I.; Sepúlveda, F.J.; Montero, I.; Romero, P.; Miranda, M.T. Feasibility Analysis of Brewers’ Spent Grain for Energy Use:Waste and Experimental Pellets. Appl. Sci. Vol. 11 (2021), p. 2740-2758.

[37] Fangyong Fan, Yusong Xie, Ning Gan, et al. Screening of Fermentation Microorganism for Distiller’s Grains[J]. Food and Fermentation Science and Technology, Vol. 58 (2022) No. 2, p. 102-106+110.

[38] Mjoun K, Kalscheur K F, Hippen A R, et al.Lactation performance and amino acid utilization of cows fed increasing amounts of reduced-fat dried distillers grains with solubles[J].Journal of Dairy Science, Vol. 93 (2010) NO. 1, p. 288-303. 

[39] Kai Zhou, Hui Xu, Jianjun Liu. Study on fermentation process of multi-enzyme probiotic feed prepared by liquor lees[J].Feed Industry, Vol. 37 (2016) No. 12, p. 42-46.

[40] Gang Yang, Diqin Yang, Xiaodan Wang, et al. A novel thermostable cellulase-producing Bacillus licheniformis A5 acts synergistically with Bacillus subtilis B2 to improve degradation of Chinese distillers' grains[J]. Bioresource technology, Vol. 325 (2021), p. 124729-124747.

[41] Mavrommatis A, Giamouri E, Myrtsi E D,et al. Antioxidant Status of Broiler Chickens Fed Diets Supplemented with Vinification By-Products: A Valorization Approach[J].Animals, Vol. 8 (2021), p. 1250-1276.

[42] Yu Z,Cheng W,Weifa S, et al. Co-fermented yellow wine lees by Bacillus subtilis and Enterococcus faecium regulates growth performance and gut microbiota in finishing pigs[J]. Frontiers in Microbiology, Vol.13 (2022), p. 1003498-1003511.

[43] Felipe A Ribeiro, Katherine I Domenech-Pérez, Carmen J Contreras-Castillo, et al. Feeding distillers grains to cattle may affect beef tenderness early postmortem.[J]. Journal of animal science, Vol. 97 (2019) No. 2, p. 445-469.

[44] S. Katsumata, Y. Hayashi, K. Oishi, T. Tsukahara, et al. Effects of liquefied sake lees on growth performance and faecal and blood characteristics in Japanese Black calves[J]. Animal, Vol. 17 (2023) No. 7, p. 100873-100889.

[45] Obeidat, Belal S. Influence of corn-dried distiller's grain with solubles on growth performance and blood metabolites of Awassi lambs offered a concentrate diet[J].Italian Journal of Animal Science, Vol.17 (2018) No. 3, p. 636-642.

[46] Gen Li, Yue Yuan, Bowen Jin, et al. Feasibility insights into the application of Paenibacillus pabuli E1 in animal feed to eliminate non-starch polysaccharides[J]. Front Microbiol, Vol. 13 (2023), p. 1205767-1205781.

[47] Genwang Han, Haojie Zhao. Prevention and treatment of pig distiller's lees poisoning[J]. Hebei Agriculture, Vol. 3 (2019), p. 56-57.

[48] Yu Zhang, Cheng Wang, Weifa Su, et al. Co-fermented yellow wine lees by Bacillus subtilis and Enterococcus faecium regulates growth performance and gut microbiota in finishing pigs[J]. Front Microbiol, Vol.13 (2022), p. 1003498-1003516.

[49] Li, Y. J., Chen, X., Kwan, T. K., et al. Dietary fiber protects against diabetic nephropathy through short-chain fatty acid-mediated activation of G protein-coupled receptors GPR43 and GPR109A. J. Am. Soc. Nephrol. Vol.31 (2022), p. 1267-1281.

[50] Yong Wang, Zhongde Li. Effects of Different Levels of Probiotic Fermentation Wine Lees on Growth Performance, Slaughtering Performance and Meat Quality of Fattening Cattle[J]. China Feed, Vol. 11 (2020), p. 117-120.

[51] Kaylie A, Huizenga, Joshua C. McCann. Investigating the Effects of Distillers Grains on Heifer Feeding Behavior in the Finishing Phase[J]. Animals (Basel), Vol.11 (2021) No. 7, p. 1905-1917.

[52] KellenB. Hart, Felipe A. Ribeiro, Morgan L. Henriott, et al. Quality effects on beef strip steaks from cattle fed high-protein corn distillers grains and other ethanol by-products[J]. J Anim Sci,Vol.97 (2019) No. 5, p. 2087-2098.

[53] Agata Bartkowiak, Joanna Lemanowicz, Magdalena Rydlewska. Enzymatic Activity of Soil after Applications Distillery Stillage[J]. Agriculture, Vol. 12 (2022) No. 5, p. 652-666.

[54] Yadong Huang, Hu Shao. Composting of distiller's grains-wheat straw bio-organic fertilizer and its application [J]. Journal of Anhui Agricultural Sciences, Vol. 37 (2009) No. 36, p. 7923-7925.

[55] Brody D. Wallis, Phillip A. Gunter, Gerald W. Horn. Replacing Fertilizer with Dried Distillers’ Grains in Stocker Cattle Systems on Southern Great Plains Old World Bluestem, USA[J]. Animals, Vol. 13 (2023) No. 18, p. 2904-2922.

[56] Lopes C L A ,Ferrari F P ,Pires C C , et al. New Paradigm for Vinasse Use as Fertilizer in Hydroponics [J]. Sugar Tech, Vol. 24 (2022) No. 4, p. 1260–1271. 

[57] Chenqin Wang, Chengxi Wei, Fan Yang. Effects of organic fertilizer of sake lees on yield and quality of sorghum [J]. Guizhou Agricultural Sciences, Vol. 40 (2012) No. 9, p. 97-100.

[58] May M L , Hands M L , Quinn M J ,et al. Dried distiller’s grains with solubles in steam-flaked or dry-rolled corn diets with reduced roughage levels[J].Kansas Agricultural Experiment Station Research Reports, 2008.

[59] Xiaobing Zhou, Pu Zheng. Fermentation of baijiu lees as raw material to produce succinic acid[J]. Food and Fermentation Industry, Vol.39 (2013) No. 2, p. 7-10.

[60] Xiaobing Zhou, Pu Zheng. Spirit-based distillers' grain as a promising raw material for succinic acid production [J]. Biotechnology Letters, Vol.35 (2013) No. 5, p. 679-684.

[61] Zanfonato, KellenSchmidt, MelodiQuines, et al. Can vinasse be used as carbon source for poly(3-hydroxybutyrate) production by cupriavidus necator DSM 545?[J].Brazilian journal of chemical engineering, Vol. 35 (2018) No. 3, p. 901-908.

[62] Eder S A, Magrini E F, Spengler A, et al. Comparison of hydrogen and volatile fatty acid production by Bacillus cereus , Enterococcus faecalis and Enterobacter aerogenes singly, in co-cultures or in the bioaugmentation of microbial consortium from sugarcane vinasse[J]. Environmental Technology & Innovation, Vol. 18 (2020) No. 6, p. 100638-100651.

[63] Braga L P P , Alves R F ,Marina T. F. Dellias. Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles[J]. Biodata Mining, Vol. 10 (2017) No. 1, p. 17-24.

[64] Banjo TT, Kareem SO, Popoola TO, Akinloye OA. Microbialproduction of ascorbic acid from brewery spent grain (BSG) byAspergillus flavus and Aspergillus tamari. Food Appl Biosci J, Vol 6 (2018) No, 2, p. 93–105

[65] Noriko A. Cassman, Késia S. Lourenço, Janaína B.do Carmo, et al. Genome-resolved metagenomics of sugarcane vinasse bacteria[J]. Biotechnology for Biofuels, Vol 11 (2018) No. 1, p. 48-63.

[66] Varga J, Baranyi N, Chandrasekaran M, et al. Mycotoxin producers in the Aspergillus genus: an update. Acta BiolSzeged, Vol 59 (2015) No. 2, p. 151–167.