Frontiers in Medical Science Research, 2024, 6(3); doi: 10.25236/FMSR.2024.060302.
Yingjia Li1, Sihua Lu1, Junyu Meng1, Changlong Hu1, Guihong Huang1,2,3,4,5
1Lingui Clinical College, Guilin Medical University, Guilin, Guangxi, 541199, China
2Department of Pharmacy, The Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, 541199, China
3Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, Key Laboratory of Diabetic Systems Medicine, Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent Medical Engineering for Chronic Diseases, Guilin, Guangxi, 541199, China
4Guangxi Key Laboratory of Drug Discovery and Optimization, Guangxi Engineering Research Center for Pharmaceutical Molecular Screening and Drug Gability Evaluation, School of Pharmacy, Guilin Medical University, Guilin, Guangxi, 541199, China
5Guangxi Clinical Research Center for Diabetes and Metabolic Diseases, Guilin, Guangxi, 541199, China
Insulin resistance is the main pathogenesis of type 2 diabetes. Previous studies have proved that isoquercitrin, a natural flavonoid extracted from Bidens bipinnata, can alleviate insulin resistance, but its mechanism is still unknown. Ring finger protein 6 (RNF6) is related to the degradation and ubiquitination of target protein, and it is a potential target for the treatment of insulin resistance. db/db mice were constructed with RNF6 overexpression, and their body weight and fasting blood glucose levels were recorded. After isoquercitrin administration, the body weight, fasting blood glucose and fasting insulin content of mice in each group were measured, and the pancreatic tissue morphology of mice in each group was observed by HE staining. The protein expressions of RNF6, IRS1 and PIK3R1 in cells were detected by Western blot. The results showed that RNF6 overexpression mice were successfully constructed. Overexpression of RNF6 would aggravate the insulin resistance of mice and inhibit the expression of IRS1 and PIK3R1, while isoquercitrin administration could reduce the expression of RNF6, up-regulate the expression of IRS1 and PIK3R1 and relieve the insulin resistance of mice. In a word, the above results indicate that isoquercitrin can target RNF6, up-regulate the expression of IRS1 and PIK3R1 decreased by RNF6, and relieve insulin resistance in db/db mice.
Insulin resistance, Isoquercitrin, Type 2 diabetic, RNF6, db/db mice
Yingjia Li, Sihua Lu, Junyu Meng, Changlong Hu, Guihong Huang. Study on Isoquercitrin Relieving Insulin Resistance of RNF6 Overexpressing db/db Mice. Frontiers in Medical Science Research (2024), Vol. 6, Issue 3: 6-12. https://doi.org/10.25236/FMSR.2024.060302.
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