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Frontiers in Medical Science Research, 2020, 2(1); doi: 10.25236/FMSR.2020.020103.

Gene Chip Detection of Gene Expression Profile Changes after Treatment of Molt-4 Cells by Smo-Sirna

Author(s)

Zhu Huamin, Bao Shijie, Lin Baixing, Sun Zhiqiang*

Corresponding Author:
Sun Zhiqiang
Affiliation(s)

Shenzhen Hospital, Southern Medical University, Guangdong Province, 518101, China
[email protected]
*Corresponding Author

Abstract

The gene expression profile of Molt-4 cells treated by Smo-siRNA was detected by gene chip, and the potential target genes regulated by Smo-siRNA were screened. The transfection group Molt- was constructed by lentiviral transfection technique. 4 cell lines. Bioinformatics analysis showed that these genes are mainly involved in cell growth and cycle, signal transduction, cell communication, cell adhesion, cell metastasis and invasion, and the signaling pathways involved antigen processing and presentation, cytokine and receptor interaction, Cell adhesion molecules, complement system, etc. Conclusions The study of potential target genes or signaling pathways regulated by Smo-siRNA is important for exploring the mechanism of Mot-4 cell development after Smo-siRNA treatment.

Keywords

Gene expression profile, Smo-sirna, Real-time pcr

Cite This Paper

Zhu Huamin, Bao Shijie, Lin Baixing, Sun Zhiqiang. Gene Chip Detection of Gene Expression Profile Changes after Treatment of Molt-4 Cells by Smo-Sirna. Frontiers in Medical Science Research (2020) Vol. 2 Issue 1: 20-27. https://doi.org/10.25236/FMSR.2020.020103.

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