The soothing and repairing effects of Artemisia annua and Centella asiatica extract composition were investigated in cell, zebrafish, and human clinical experiments. The effects of the composition on the secretion of TNF-α from RAW264.7 (mouse macrophages) were studied in cell experiments, and the effects of the composition on the proliferation of HCEC (human corneal epithelial cells) and HaCaT (human immortalized epidermal cells) were also studied. The repairing effect of the composition was verified by zebrafish experiment. In the human clinical experiment, the changes in skin transdermal water loss TEWL value and skin redness a* in volunteers before and after using the gel of the composition were tested. The results showed that the composition had no significant stimulating effect on HCEC, but significantly inhibited the secretion of TNF-α by RAW cells. The composition significantly promoted the migration of HaCaT cells and the repairing of zebrafish tail fins. The human clinical experiment showed that the rate of change of skin transdermal water loss TEWL value of the gel was -17.95% (P<0.001), and skin redness was significantly reduced (P<0.01). In conclusion, the combination of Artemisia annua and Centella asiatica extract has soothing and repairing effects.

Daohuan Yuan, Jiamin Huang, Ji Lin, Jiajia Tian, Xiaojun Cai, Rui He, Yong Xie. Study on the Soothing and Repairing Effects of Artemisia Annua Combined with Centella Asiatica Extract. Frontiers in Medical Science Research (2024), Vol. 6, Issue 7: 9-16. https://doi.org/10.25236/FMSR.2024.060702.

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