Academic Journal of Materials & Chemistry, 2025, 6(2); doi: 10.25236/AJMC.2025.060206.
Hongqu Liang1, Liu Huang1, Peng Yan1, Fa Huang2, Hongchao Zhang2, Qisheng Luo1
1The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China
2Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Dopamine (DA) is one of the key neurotransmitters (NTs) in nature and plays a crucial role in the mammalian central nervous system (CNS). Its selective determination in biological fluids is a fundamental need in the field of biomedical research. In this study, a novel electrochemical biosensor was developed, taking advantage of the high selectivity and specificity of the CRISPR/Cas12 system. The aptamer sensor was constructed and characterized using cyclic voltammetry with a glassy carbon electrode (GCE), and its conductivity was demonstrated by electrochemical impedance spectroscopy (EIS). The sensor exhibited a good linear response in the range of 1–10 μM, with a detection limit of 4.98 μmol/L. Compared to conventional methods, the electrochemical sensor developed in this study shows high sensitivity and excellent selectivity. The developed sensor was applied to the determination in analogous samples, yielding satisfactory recovery results.
CRISPR/Cas12 system, glassy carbon electrode, dopamine assay, electrochemical biosensor
Hongqu Liang, Liu Huang, Peng Yan, Fa Huang, Hongchao Zhang, Qisheng Luo. Combined CRISPR/Cas12 system and glassy carbon electrode enable detection and analysis of dopamine solution. Academic Journal of Materials & Chemistry (2025), Vol. 6, Issue 2: 43-50. https://doi.org/10.25236/AJMC.2025.060206.
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