Yajie Fan, Zhihui Yao, Tuo Han, Lixia Wang, Yiwen Wang, Congxia Wang
Department of Cardiovascular Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China
Background: Ischemic cardiomyopathy (ISCM) following myocardial infarction is closely related with a poor prognosis in patients with coronary heart disease. Several researches have showed that noncoding RNAs, including long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) are involved in the progression of ISCM. The aim of the present study was to explore the potential biomarkers and competing endogenous RNA (ceRNA) machenism in ISCM. Methods: Based on the open-source database, we identified differentially expressed genes (DEGs) and differentially expressed lncRNAs (DELs) between samples from ISCM patients and controls using affy and limma package in R (absolute log2 fold change ≥1 and p<0.05). We prediced target miRNAs of DELs and DEGs by TargetScanHuman, miRDB and miRTarBase with at least two validations in three databases. Gene ontology (GO) analysis of DEGs was performed by clusterProfiler package in R. Taken the nodes together, we tried to build a net according to ceRNA theory. Results: We integrated the crosstalk of DEGs, DELs and miRNAs and constructed a ceRNA network. There are 21 lncRNAs, 39 miRNAs, and 41 mRNAs involved in the network. Functional analysis showed that DEGs were most prominently associated with molecular functions in extracellular matrix. Conclusion: By constructing ceRNA net in ISCM, we found that several important lncRNAs and genes were included. More nodes need to be verified both in vivo and in vitro.
Ischemic cardiomyopathy (ISCM), Long nongcoding RNA (lncRNA), Bioinformatics, Competing endogenous RNA (ceRNA), Cardiovascular disease
Yajie Fan, Zhihui Yao, Tuo Han, Lixia Wang, Yiwen Wang, Congxia Wang. The Role of Competing Endogenous RNA (ceRNA) Network in Ischemic Cardiomyopathy by Bioinformatic Analysis. Frontiers in Medical Science Research (2021) Vol. 3 Issue 2: 29-36. https://doi.org/10.25236/FMSR.2021.030208.
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