Considering the requirements of high precision, automation, fast speed and batch production for sample injection detection by fluorescence spectrophotometer, a fluorescence automatic sampling instrument for drug toxicity detection based on the information angle of binding with the carrier protein are developed for automatic sample injection. Firstly, through the "carrier protein binding information-toxicity relationship", the fluorescence automatic sampling instrument is controlled online to realize automatic sampling and online collection of spectral signals. Then, based on the changes in the manual sampling of the traditional fluorescence spectrophotometer, a hands-free and airtight sampling method was proposed to avoid measurement errors caused by manual sampling. Next, Sterilization thermostats and temperature regulators are added to ensure that the samples are not cross-contaminated and reduce the rate of protein deformation. Finally, using the "carrier protein binding information-toxicity relationship" to control sample injection in real-time, predict the acute toxicity LD50 and toxicity level of the sample, and provide suggestions for modification of the test substance structure. The fluorescence automatic sampling instrument for drug toxicity detection based on the information angle of binding to the carrier protein is suitable for online collection and analysis of large quantities of drug toxicity.

Xiangshuai Li, Xiangfen Li, Zishi Wang, Hongliang Xu. Fluorescence Automatic Sampling Instrument for Drug Toxicity Detection Based on the Information Angle of Binding with the Carrier Protein. Academic Journal of Engineering and Technology Science (2021) Vol. 4, Issue 4: 41-48. https://doi.org/10.25236/AJETS.2021.040408.

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