Frontiers in Medical Science Research, 2021, 3(5); doi: 10.25236/FMSR.2021.030504.
Hang Fan1, Yingli Cao2, Ziming Xi3, Guoying Wang4, Jia Zheng1
1Department of Orthopedics, Henan University People's Hospital, Henan Provincial People's Hospital, Henan Zhengzhou, 450003, China
2School of Medical, Nankai University, Tianjin, 300192, China
3The First Affiliated Hospital of Henan University, Henan Kaifeng, 475001, China
4School of Basic Medicine Science, Henan University, Henan Kaifeng 475004, China
Objective: To investigate the protective effect of Panax ginseng on osteoporosis in guinea-pigs induced by ovariectomy. Methods: Six-month-old 36 SPF non-pregnant female guinea-pigs, were divided into four groups randomly: sham-operated group (Sham), blank model group (Model), recommended dose of Panax ginseng group (RD) and Maximum dose of Panax ginseng group (MD), 9 guinea-pigs in each group. Guinea-pigs models of osteoporosis were prepared by ovariectomized (OVX), except Sham group. 2 weeks later, OVX guinea-pigs were administered gavage with Panax ginseng powder twice daily for 100 days, RD group with 2.5mg/100gBW, MD group with 5mg/100 gBW. Sham and Model group were given with the same dose of saline, all the gavage dose were 3mL. The serum of guinea-pigs were obtained to measure the electrolytes ions, serum Ca2+, Mg2+, P3+, to measure serum bone metabolism indexes BALP, OC, TRACP-5b, BMD and BMC. Results: Compared with Model group, the level of serum Ca2+, P3+ decreased in the Panax ginseng groups, but the level of Mg2+increased (P<0.05), the statistics difference in MD group was significant (P<0.01). The influence of serum BALP, OC, and TRACP-5b: compared with Model group, the Panax ginseng groups had statistical differences (P<0.05), with the dose correlated positively. Compared with Model group, the index values of BMD and BMC of the Panax ginseng groups were significantly Maximum (P<0.05), there was a dose-dependent difference with drug intervention. Conclusion: Panax ginseng can inhibit bone loss, decrease bone turnover rate, and improve the osteoporosis with dose-dependent effect.
Panax ginseng, osteoporosis, guinea-pigs, ovariectomy
Hang Fan, Yingli Cao, Ziming Xi, Guoying Wang, Jia Zheng. Protective Effect of Panax ginseng on osteoporosis in Ovariectomized Female Guinea-pigs. Frontiers in Medical Science Research (2021) Vol. 3 Issue 5: 24-29. https://doi.org/10.25236/FMSR.2021.030504.
[1] S YEDAVALLY-YELLAYI, AM HO, EM PATALINGHUG. Update on Osteoporosis. Primary care 2019 Mar; 46(1): 175-190.
[2] FU LJ, MA JH, YAN SM, et al. A meta-analysis of VDR polymorphisms and postmenopausal osteoporosis [J]. Endocrine connections 2020 10; 9(9): 882-889.
[3] LIN XZ, LI L, WU SL, et al. Activation of GPR30 promotes osteogenic differentiation of MC3T3-E1 cells: An implication in osteoporosis [J]. IUBMB life 2019 11; 71(11): 1751-1759.
[4] S ROZENBERG, N AL-DAGHRI, M AUBERTIN-LEHEUDRE, et al. Is there a role for menopausal hormone therapy in the management of postmenopausal osteoporosis? [J].Osteoporosis international 2020 Dec; 31(12): 2271-2286.
[5] GH RYOO, YJ MOON, SH CHOI, et al. Tussilagone promotes osteoclast apoptosis and prevents estrogen deficiency-induced osteoporosis in mice. Biochemical and biophysical research communications 2020 10 22; 531(4): 508-514.
[6] J A KANIS, C COOPER, R RIZZOLI, et al. Scientific Advisory Board of the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) and the Committees of Scientific Advisors and National Societies of the International Osteoporosis Foundation (IOF); Executive summary of the European guidance for the diagnosis and management of osteoporosis in postmenopausal women [J]. Calcified tissue international 2019 03; 104(3): 235-238.
[7] DS IM. Pro-Resolving Effect of Ginsenosides as an Anti-Inflammatory Mechanism of Panax ginseng [J]. Biomolecules 2020 03 13; 10(3).
[8] JL LEE, KY SUN PARK, IKH CHO. Panax ginseng: a candidate herbal medicine for autoimmune disease [J]. Journal of ginseng research 2019 Jul; 43(3): 342-348.
[9] KO OCHIAI, KJ KAWASAKI. Panax ginseng for Frailty-Related Disorders: A Review. Frontiers in nutrition 2018; 5: 140.
[10] GF LIU, ZQ WANG, L LIU, et al. A network meta-analysis on the short-term efficacy and adverse events of different anti-osteoporosis drugs for the treatment of postmenopausal osteoporosis [J]. Journal of cellular biochemistry 2018 06; 119(6): 4469-4481.
[11] B ERHAN, Y ATAKER. Rehabilitation of Patients with Osteoporotic Fractures [J]. Journal of clinical densitometry 2020 Oct - Dec; 23(4): 534-538.
[12] MCCREADY J, BEAUFRÈRE H, SINGH A, et al. Laparoscopic ovariectomy in guinea pigs: A pilot study [J]. Vet Surg, 2020, 1:131-137.
[13] BOKHARI R A, LAU S F, MOHAMED S. Orthosiphon stamineus (Misai Kucing) ameliorated postmenopausal osteoporosis in rat model [J]. Menopause, 2018, 25(2): 202-210.
[14] ZHANG Z, ZHAO Q, LIU T, et al. Effect of vicenin-2 on ovariectomy-induced osteoporosis in rats [J]. Biomed Pharmacother, 2020, 129: 110474.
[15] ZHENG H, PAN L, LYU J, et al. Comparison of immune responses in guinea pigs by intranasal delivery with different nanoparticles-loaded FMDV DNA vaccine[J]. Microb Pathog, 2020, 142: 104061.
[16] J A RAJABIAN, M RAMESHRAD, H HOSSEINZADEH. Therapeutic potential of Panax ginseng and its constituents, ginsenosides and gintonin, in neurological and neurodegenerative disorders: a patent review. Expert opinion on therapeutic patents 2019 01; 29(1): 55-72
[17] Wang LJ, Huang Y, GuoY, et al. Antimicrobial activities of Asian ginseng, American ginseng, and notoginseng [J]. Phytotherapy research: PTR, 2020, 34(6): 1226-1236.
[18] HAO M, ZHOU Y, ZHOU J, et al. Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process [J]. J Ginseng Res, 2020, 44(5): 747-755.