Juanjuan Zhang1,2,3,＃, Weizhe Liu4,＃, Hidekatsu Yoshioka5, Rong Wang3, Yanqing Zhang3, Yongqing Shen2,3, Aiying Li1
1Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China
2Affiliation Key Laboratory for Health Care with Chinese Medicine of Hebei Province, School of Nursing, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China
3College of Nursing, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China
4Department of Clinical Foundation of Chinese Medicine, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China
5Department of Matrix Medicine, Faculty of Medicine, Oita University, Oita, Japan
＃These authors contributed equally to this work (co-first-author)
Type XIX collagen is an extremely rare extracellular matrix collagen thought to be involved in the formation of basement membrane zones and is transiently expressed by differentiating muscle cells. Mice without collagen XIX exhibit an impaired muscle differentiation and function. Individual skeletal muscle fibers are ensheathed by a meshwork of collagen fibers comprising the endomysium. Such structures seem to play an important role in resisting imputes. Type XIX collagen-null mice have a poor survival, and surviving null mice exhibit a dilated esophagus after three months of age, especially in aged mice. We used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to examine the skeletal muscle morphology and reticular distribution of collagen fibers in the endomysium of the esophagus in mice. The aim of this research was to demonstrate the fine structures in the dilated portion of the esophagus in mutant mice. Our findings showed that the size and arrangement of skeletal muscle as well as the collagen fibrils of the endomysium in the dilated portion of the esophagus differed markedly from those in wild-type mice. In addition, skeletal abnormalities were seen in the non-dilated portion of the esophagus in the mutant mice. These findings suggest that type XIX collagen-containing fibrils influence morphogenesis during skeletal myogenesis in the murine esophagus.
type XIX collagen, mutant mouse, skeletal muscle, esophagus, achalasia, basement membrane
Juanjuan Zhang, Weizhe Liu, Hidekatsu Yoshioka, Rong Wang, Yanqing Zhang, Yongqing Shen, Aiying Li. Impairment of esophageal skeletal muscle in type XIX collagen mutant mouse. Frontiers in Medical Science Research (2022) Vol. 4, Issue 6: 52-59. https://doi.org/10.25236/FMSR.2022.040610.
 Rowe, R. (1981) Morphology of perimysial and endomysial connective tissue in skeletal muscle. Tissue Cell, 13, 681 - 690.
 Ohtani, O., Ushiki, T., Taguchi, T., Kikuta, A. (1988) Collagen fibrillar networks as skeletal frameworks: a demonstration by cell-maceration/scanning electron microscope method. Arch Histol Cytol, 51, 249 - 261.
 Morita, T., Shimada, T., Kitamura, H., Nakamura, M. (1991) Demonstration of connective tissue sheaths surrounding working myocardial cells and purkinje cells of the sheep moderator band. Arch Histol Cytol, 54, 539 - 550.
 Masutomo, K., Makino, N., Maruyama, T., Shimada, T., Yanaga, T. (1996) Effects of enalapril on the collagen matrix in cardiomyopathic Syrian hamsters (Bio 14.6 and 53.58). Jpn Circ J, 60, 50 - 61.
 Patapoutian, A., Wold, B.J., Wagner, R.A. (1995) Evidence for developmentally programmed transdifferentiation in mouse esophageal muscle. Science, 270, 1818 - 1821.
 Worl, J, Neuhuber, W.L. (2005) Ultrastructural analysis of the smooth-to-striated transition zone in the developing mouse esophagus: Emphasis on apoptosis of smooth and origin and differentiation of striated muscle cells. Dev Dyn, 233, 964 - 982.
 Krauss, R.S., Chihara, D., Romer, A.I. (2016) Embracing change: striated-for-smooth muscle replacement in esophagus development. Skeletal muscl, 6, 27.
 Myers, J.C., Li, D., Bageris, A., Abraham, V., Dion, A.S., Amenta, P.S. (1997) Biochemical and immunohistochemical characterization of human type XIX defines a novel class of basement membrane zone collagens. Am J pathol, 151, 1729 - 1740.
 Sumiyoshi, H., Mor, N., Lee, S.Y., Doty, S., Henderson, S., Tanaka, S., Yoshioka, H., Rattan, S., Ramirez, F. (2004) Esophageal muscle physiology and morphogenesis require assembly of a collagen XIX-rich basement membrane zone. J Cell Biol, 166, 591 - 600.
 Montes, G.S., Krisztan, R.M., Shigihara, K.M., Tokoro, R., Mourao, P.A. Junqueira (1980) Histochemical and morphological characterization of reticular fibers. Histochemistry, 65, 131-141.
 Gordon, M.K., Hahn, R.A. (2010) Collagens. Cell Tissue Res, 339, 247 - 257.
 Svoboda, K.K., Nishimura, I., Sugrue, S.P., Ninomiya, Y., Olsen, B.R. (1988) Embryonic chicken cornea and cartilage synthesize type IX collagen molecules with different amino-terminal domains. Proc Natl Acad Sci USA, 85, 7496 - 7500.
 Walchli, C., Koch, M., Chiquet, M., Odermatt, B.F., Trueb, B. (1994) Tissue-specific expression of the fibril-associated collagen XII and XIV. J Cell Sci, 107 (Pt2), 669 - 681.
 Gockel, H.R., Schumacher, J., Gockel, I., Lang, H., Haaf, T., Nothen, M.M .(2010) Achalasia: will genetic studies provide insight? Hum Genet, 128, 353 - 364.
 Gockel, I., Bohl, J.R., Eckardt, V.F., Junginger, T. (2008) Reduction of interstitial cells of Cajal (ICC) associated with neuronal nitric oxide synthase (n-NOS) in patients with achalasia. Am J Gastroenterol, 103, 856 - 864.