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Frontiers in Medical Science Research, 2022, 4(8); doi: 10.25236/FMSR.2022.040806.

Antibacterial Activity and Mechanism of Aureusidin Against Staphylococcus Aureus


Jie Ren, Jianyin Zhou, Wenzheng Zhang, Jichao Zhou, Shaojun Wu

Corresponding Author:
​Jie Ren

School of Pharmacy, Changzhou University, 213164, Changzhou, China


Aureusidin is a novel compound with antibacterial activity against Staphylococcus aureus (S. aureus), yet the mechanism of aureusidin against S. aureus has not been completely described. The objective of this study aimed at evaluating the inhibition of the growth of S. aureus, investigating the antibacterial activity and mechanism of action of aureusidin against S. aureus. The mechanism of action of aureusidin was investigated by analysing its effects on the cell morphology, the membrane permeability, the electrical conductivity, the nucleic acid leakage and the protein release at MIC. The respiratory rate of S. aureus decreased when the concentration of aureusidin was increased to MIC, and was the same as malonic acid. This inferred that the TCA cycle was restrained, reducing the energy transfer of S. aureus and inhibiting the growth of S. aureus. Thus, these results showed that aureusidin had a strong antibacterial effect on inhibiting the growth of S. aureus and provided application potential in the field on food preservatives.


Aureusidin; Staphylococcus aureus; MIC; Bacteriostatic activity; Bacteriostatic mechanism

Cite This Paper

Jie Ren, Jianyin Zhou, Wenzheng Zhang, Jichao Zhou, Shaojun Wu. Antibacterial Activity and Mechanism of Aureusidin Against Staphylococcus Aureus. Frontiers in Medical Science Research (2022) Vol. 4, Issue 8: 24-31. https://doi.org/10.25236/FMSR.2022.040806.


[1] Wang Z. and Yang L. (2021) Chinese herbal medicine: Fighting SARS-CoV-2 infection on all fronts. J Ethnopharmacol, 270, 113869.

[2] Xu Y.X.Z., Xi S. and Qian X. (2019) Evaluating Traditional Chinese Medicine and Herbal Products for the Treatment of Gestational Diabetes Mellitus. J Diabetes Res, 2019, 9182595.

[3] Wu X., Hu X., Zhang Q., Liu F. and Xiong K. (2021) Regulatory Role of Chinese Herbal Medicine in Regulated Neuronal Death. CNS Neurol Disord Drug Targets, 20, 228-248.

[4] Yang Y., Sheng Y., Wang J., Zhou X., Guan Q., Shen H., Li W. and Ruan S. (2021) Aureusidin derivative CNQX inhibits chronic colitis inflammation and mucosal barrier damage by targeting myeloid differentiation 2 protein. J Cell Mol Med, 25, 7257-7269.

[5] Ren J., Su D., Li L., Cai H., Zhang M., Zhai J., Li M., Wu X. and Hu K. (2019) Anti-inflammatory effects of Aureusidin in LPS-stimulated RAW264.7 macrophages via suppressing NF-κB and activating ROS- and MAPKs-dependent Nrf2/HO-1 signaling pathways. Toxicol Appl Pharmacol, 387, 114846.

[6] Yang Y., Han C., Sheng Y., Wang J., Zhou X., Li W. and Guo L. (2020) The Mechanism of Aureusidin in Suppressing Inflammatory Response in Acute Liver Injury by Regulating MD2. Front Pharmacol, 11, 570776.

[7] McGuinness W.A., Malachowa N. and DeLeo F.R. (2017) Vancomycin Resistance in Staphylococcus aureus. Yale J Biol Med, 90, 269-281.

[8] Oliveira D., Borges A. and Simões M. (2018) Staphylococcus aureus Toxins and Their Molecular Activity in Infectious Diseases. Toxins (Basel), 10, 252.

[9] Lee A.S., Lencastre H., Garau J., Kluytmans J., Malhotra-Kumar S., Peschel A. and Harbarth S. (2018) Methicillin-resistant Staphylococcus aureus. Nat Rev Dis Primers, 4, 18033.

[10] Kwiecinski J.M. and Horswill A.R. (2020) Staphylococcus aureus bloodstream infections: pathogenesis and regulatory mechanisms. Curr Opin Microbiol, 53, 51-60.

[11] Becker K., Schaumburg F., Kearns A., Larsen A.R., Lindsay J.R., Skov R.L. and Westh H. (2019) Implications of identifying the recently defined members of the Staphylococcus aureus complex S. argenteus and S. schweitzeri: a position paper of members of the ESCMID Study Group for Staphylococci and Staphylococcal Diseases (ESGS). Clin Microbiol Infect, 25, 1064-1070.

[12] Espiritu R.A. (2021) Repairing plasma membrane damage in regulated necrotic cell death. Mol Biol Rep, 48, 2751-2759.

[13] Murati T., Miletić M., Pleadin J., Šimić B. and Kmetič I. (2020) Cell membrane-related toxic responses, 40, 1592-1601.