Nuclear factor Y (NF-Y) is a heterotrimeric, DNA-binding transcription factor (TF) that is conserved in all eukaryotes, which consists of three subunits, NF-YA, NF-YB, and NF-YC, and binds to the CCAAT box in the promoter regions of its target genes to regulate their expression. AtNF-YB8, a transcription factor from Arabidopsis thaliana, plays an important role in many biological processes in plants. In order to understand the structure and DNA binding model of AtNF-YB8, the 3D structure model of AtNF-YB8 was constructed and docked with the eleven target DNA. Also, the decomposition of the binding energy on a per-residue basis in the AtNF-YB8/DNA07 complex by MD simulations showed that the residues Lys-34, Lys-96, Lys-101 and Lys-110 were probably for specific recognition of DNA.

Jubaili Norah Ahmed H, Liang-jianLi, Rui Hu, Wan-Jun wang. In silico analysis on structure and DNA binding  mode of AtNF -YB8 in Arabidopsis thaliana L. Frontiers in Medical Science Research (2019) Vol. 1 Issue 2: 1-14. https://doi.org/10.25236/FMSR.20190201.

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