Frontiers in Medical Science Research, 2022, 4(14); doi: 10.25236/FMSR.2022.041401.
Junze Zhao, Jiawei Wang, Jianhao Wang
School of Pharmacy, Changzhou University, Changzhou, 213164, China
The discovery of broad-spectrum antibiotics has greatly reduced human mortality due to bacterial infections, but the widespread use of broad-spectrum antibiotics in the past 20 years has led to the emergence of multiple drug resistance (MDR) pathogens, making bacterial infection modern serious problems facing medicine. In addition, the abuse of broad-spectrum antibiotics can also cause diseases such as intestinal flora imbalance, colitis, and candidiasis. Therefore, it is of great significance to develop new targeted antibacterial drugs, which can effectively treat bacterial infections while reducing the generation of drug-resistant bacteria and avoiding the imbalance of microbiota in patients. Based on this, we designed a temperature-responsive nano-drug delivery system containing antimicrobial peptides. The system uses gold nanostars as carriers to couple targeted antimicrobial peptides and loads them into thermosensitive hydrogels. It can form a gel according to the increase in the ambient temperature, and the photothermal effect is stable. In vitro antibacterial experiments show that the system has an excellent killing ability for StaphylococcuS.aureus. The method reported in this article hopes to provide a certain reference value for future nanoparticle antibacterial research.
Targeted Peptide Nanodrug, Photothermal therapy, Temperature sensitive hydrogel
Junze Zhao, Jiawei Wang, Jianhao Wang. Construction and Antibacterial Study of Targeted Peptide Nanodrug Delivery System. Frontiers in Medical Science Research (2022) Vol. 4, Issue 14: 1-8. https://doi.org/10.25236/FMSR.2022.041401.
 Mulani MS, Kamble EE, Kumkar SN, Tawre MS, Pardesi KR. Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review. Front Microbiol. 2019; 10: 539.
 Bush K, Bradford P A. β-Lactams and β-lactamase inhibitors: an overview [J]. Cold Spring Harbor perspectives in medicine, 2016, 6(8): a025247.
 Bengtsson B., Greko C. Antibiotic resistance—Consequences for animal health, welfare, and food production. Upsala J. Med. Sci. 2014; 119: 96–102.
 Mulani M.S., Kamble E.E., Kumkar S.N., Tawre M.S., Pardesi K.R. Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review. Front. Microbiol. 2019; 10: 539.
 Bush K., Bradford P.A. β-Lactams and β-Lactamase Inhibitors: An Overview. Cold Spring Harb. Perspect. Med. 2016; 6: a025247.
 Lambert T. Antibiotics that affect the ribosome. Rev Sci Tech. 2012; 31(1): 57-64.
 Shaikh S, Fatima J, Shakil S, Rizvi SM, Kamal MA. Antibiotic resistance and extended spectrum beta-lactamases: types, epidemiology and treatment. Saudi J Biol Sci. 2015; 22(1): 90–101.
 Munita JM, Arias CA. Mechanisms of Antibiotic Resistance. Microbiol Spectr. 2016; 4(2): 10.1128
 Miquel S, Lagrafeuille R, Souweine B, Forestier C. Anti-biofilm Activity as a Health Issue. Front Microbiol. 2016; 7: 592.
 Hengzhuang W, Wu H, Ciofu O et al. Pharmacokinetics/pharmacodynamics of colistin and imipenem on mucoid and nonmucoid Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother 2011; 55 (9): 4469–4474.
 Hengzhuang W, Wu H, Ciofu O et al. In vivo pharmacokinetics/pharmacodynamics of colistin and imipenem in Pseudomonas aeruginosa biofilm infection. Antimicrob Agents Chemother 2012; 56 (5): 2683–2690.
 Herrmann G, Yang L, Wu H et al. Colistin–tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. J Infect Dis 2010; 202 (10): 1585–1592.
 Amina SJ, Guo B. A Review on the Synthesis and Functionalization of Gold Nanoparticles as a Drug Delivery Vehicle. Int J Nanomedicine. 2020; 15: 9823-9857.
 Wang H, Wang M, Xu X, et al. Multi-target mode of action of silver against Staphylococcus aureus endows it with capability to combat antibiotic resistance [published correction appears in Nat Commun. 2021 Jul 1; 12(1): 4140]. Nat Commun. 2021; 12(1): 3331.
 Liang Y, Zhang J, Quan H, et al. Antibacterial Effect of Copper Sulfide Nanoparticles on Infected Wound Healing. Surg Infect (Larchmt). 2021; 22(9): 894-902.
 Chouirfa H, Bouloussa H, Migonney V, Falentin-Daudré C. Review of titanium surface modification techniques and coatings for antibacterial applications. Acta Biomater. 2019; 83:37-54.
 Yao S, Chi J, Wang Y, Zhao Y, Luo Y, Wang Y. Zn-MOF Encapsulated Antibacterial and Degradable Microneedles Array for Promoting Wound Healing. Adv Healthc Mater. 2021; 10(12); e2100056.
 Eckert R., Sullivan R., Shi W. Targeted antimicrobial treatment to re-establish a healthy microbial flora for long-term protection [J]. Adv. Dent. Res, 2002, 24: 94–97.
 Kucisec-Tepes N. ULOGA ANTISEPTIKA I STRATEGIJA UKLANJANJA BIOFILMA KRONICNE RANE THE ROLE OF ANTISEPTICS AND STRATEGY OF BIOFILM REMOVAL IN CHRONIC WOUND [J]. Acta Med Croatica, 2016 Mar; 70(1): 33-42.
 Fjell C D, Hiss J A, Hancock R E W, et al. Designing antimicrobial peptides: form follows function [J]. Nature reviews Drug discovery, 2012, 11(1): 37-51.
 Paladini F, Pollini M, Sannino A, et al. Progress and perspectives in the management of wound infections [J]. Wound Healing: New Insights into Ancient Challenges, 2016: 435-56.