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Academic Journal of Materials & Chemistry, 2023, 4(2); doi: 10.25236/AJMC.2023.040202.

Determination of D-limonene content of volatile oil clathrates by HPLC


Jiaqin Zheng1, Miao Li1, Shuai Zhang1, Xuhui Xu1, Zibo Dong1,2

Corresponding Author:
Zibo Dong

1School of Pharmacy, Jiangsu Ocean University, Lianyungang, China

2Marine Pharmaceutical Resources Development Engineering Research Center, Lianyungang, China


Aim The method of D-limonene of Fructus Aurantii Immaturus volatile oil clathrates (clathrates) was established and the solubility of clathrates was determined. Methods: The detection method of clathrates was HPLC, and the solubility of clathrates was determined by equilibrium method. Results Welch UItimate XB-C18 chromatographic column (4.6 mm×250 mm, 5 μm); The detection wavelength: 205 nm; column temperature: 35℃; sample size: 10 μL; mobile phase: methyl cyanide (A)-water (B); current speed: 1 mL/min, gradient elution program: 0~10 min 65%~75% ( A); 10~28 min 75%~78% (A); 28~32 min 78%~65% (A); 32~42 min 65% (A). The solubility of the clathrates in water is 12.75 times that of the volatile oil. Conclusion HPLC method of D-limonene content and inclusion rate is accurate and reliable, it can be used as the method to measuring the rate of clathrates. And this experimental method is stable and reliable, can prepare the clathrates with good solubility and stable properties.


D-limonene; hydroxypropyl-β-cyclodextrin; clathrates; HPLC; solubility

Cite This Paper

Jiaqin Zheng, Miao Li, Shuai Zhang, Xuhui Xu, Zibo Dong. Determination of D-limonene content of volatile oil clathrates by HPLC. Academic Journal of Materials & Chemistry (2023) Vol. 4, Issue 2: 6-10. https://doi.org/10.25236/AJMC.2023.040202.


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